PXD044467 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | CryoEM reveals that ribosomes in microsporidian spores are locked in a dimeric hibernating state |
| Description | Translational control is an essential process for the cell to adapt to varying physiological or environmental conditions. To survive adverse conditions such as low nutrient levels, translation can be shut down almost entirely by inhibiting ribosomal function. Here we investigated eukaryotic hibernating ribosomes from the microsporidian parasite Spraguea lophii in situ by a combination of electron cryo-tomography (cryoET) and single particle cryoEM. We show that microsporidian spores contain hibernating ribosomes that are locked in the 100 S dimeric state, which is formed by a unique dimerisation mechanism involving the beak region. The ribosomes within the dimer are fully assembled, suggesting that they are ready to be activated once the host cell is invaded. This study provides structural evidence for dimerisation acting as a mechanism for ribosomal hibernation in microsporidia, and therefore demonstrates that eukaryotes utilise this mechanism in translational control. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-01 |
| AnnouncementXML | Submission_2025-12-01_07:12:07.336.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rebecca Conners |
| SpeciesList | scientific name: Spraguea lophii 42_110; NCBI TaxID: NEWT:1358809; |
| ModificationList | L-methionine removal; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-08-09 10:19:22 | ID requested | |
| ⏵ 1 | 2025-12-01 07:12:07 | announced | |
Publication List
Keyword List
| submitter keyword: ribosome, Spraguea lophii,Microsporidia |
Contact List
| Bertram Daum |
|---|
| contact affiliation | Living Systems Institute Faculty of Health and Life Sciences University of Exeter UK |
| contact email | b.daum2@exeter.ac.uk |
| lab head | |
| Rebecca Conners |
|---|
| contact affiliation | University of Exeter |
| contact email | r.conners@exeter.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044467
- Label: PRIDE project
- Name: CryoEM reveals that ribosomes in microsporidian spores are locked in a dimeric hibernating state
