PXD044457-1

PXD044457 is an original dataset announced via ProteomeXchange.

Title	Cytoplasmic sequestering of a fungal stress-activated MAPK in response to a host plant phenolic acid
Description	The stress-activated MAPK Hog1 in Cochliobolus heterostrophus, a maize foliar pathogen, undergoes dephosphorylation upon exposure to ferulic acid (FA), a phenolic compound abundant in the host plant. Unlike its nuclear localization during osmotic stress, Hog1:GFP forms cytoplasmic foci in response to FA, indicating its sequestering. By using Hog1:GFP as an affinity purification bait, we isolated an FA-dependent sub-proteome from a subcellular fraction enriched with fluorescent foci. The identified proteins include RNA-binding proteins, translation initiation factors and mitochondrial proteins, suggesting the foci to be stress granules.
HostingRepository	PRIDE
AnnounceDate	2025-10-22
AnnouncementXML	Submission_2025-10-22_06:20:55.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rina Zuchman
SpeciesList	scientific name: Bipolaris maydis victorivirus 1b; NCBI TaxID: NEWT:2737160;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-08 15:38:13	ID requested
⏵ 1	2025-10-22 06:20:56	announced

Dataset with its publication pending

submitter keyword: Stress response, Stress granules,MAPK, Fungi

Benjamin Horwitz
contact affiliation	Faculty of Biology Institute Technion-Israel Institute of Technology Haifa 3109601 ISRAEL
contact email	horwitz@technion.ac.il
lab head
Rina Zuchman
contact affiliation	Technion
contact email	rinaz@technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044457
     1. Label: PRIDE project
     2. Name: Cytoplasmic sequestering of a fungal stress-activated MAPK in response to a host plant phenolic acid