PXD044361-2

PXD044361 is an original dataset announced via ProteomeXchange.

Title	PKG-dependent phosphoproteome of T. gondii treated with the phosphodiesterase inhibitor zaprinast
Description	Apicomplexans are ubiquitous parasites of humans and cause diseases including toxoplasmosis, malaria, and cryptosporidiosis. Protein kinase G (PKG) possesses unique features in this phylum of parasites and is a demonstrated drug target. Phosphodiesterase inhibitors increase the concentration of cGMP in cells, thereby activating PKG. Here, we generate a phosphoproteome from parasites treated with the phosphodiesterase inhibitor zaprinast. We additionally use an auxin-inducible degron system to deplete parasites of PKG during this treatment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:49:39.341.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044361
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alice Herneisen
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379;
ModificationList	methylthiolated residue; phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-04 11:04:02	ID requested
1	2024-10-08 11:39:04	announced
⏵ 2	2024-10-22 06:49:39	announced	2024-10-22: Updated project metadata.

10.6019/PXD044361;

10.7554/ELIFE.93877.1;

submitter keyword: Toxoplasma, PKG, parasite, T. gondii, phosphoproteomics, protein kinase G

Sebastian Lourido
contact affiliation	Associate Professor, Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology
contact email	lourido@wi.mit.edu
lab head
Alice Herneisen
contact affiliation	Whitehead Institute for Biomedical Research, Cambridge, MA, USA 02142 Department of Biology, Massachusetts Insitute of Technology
contact email	alice@wi.mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD044361

PRIDE project URI

• PRIDE
  1. PXD044361
     1. Label: PRIDE project
     2. Name: PKG-dependent phosphoproteome of T. gondii treated with the phosphodiesterase inhibitor zaprinast