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PXD044325-1

PXD044325 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEffect of an immune challenge and feed supplements on broiler chicken individual breast muscle protein synthesis
DescriptionOptimization of broiler chicken breast muscle protein accretion is key for the efficient production of poultry meat, whose demand is steadily increasing. In a context where antimicrobial growth promoters use is being restricted, it is important to find alternatives as well as to characterize the effect of immunological stress on broiler chicken growth. Despite of its importance, research on broiler chicken muscle protein dynamics has been mostly limited to the study of mixed protein turnover. The present study aims to characterize the effect of a bacterial challenge and the feed supplementation of a citrus and a cucumber extract on broiler chicken individual breast muscle proteins fractional synthesis rates (FSR) using a recently developed dynamic proteomics pipeline. 21 day-old broiler chickens were administered a single 2H2O dose before being culled at different timepoints. A total of 60 breast muscle protein extracts from five experimental groups (Unchallenged, Challenged, Control Diet, Diet 1 and Diet 2) were analyzed using a DDA proteomics approach. Proteomics data was filtered in order to reliably calculate multiple proteins FSR making use of a newly developed bioinformatics pipeline. Broiler breast muscle proteins FSR uniformly decreased following a bacterial challenge, this change was judged significant for 15 individual proteins, the two major functional clusters identified as well as for mixed breast muscle protein. Citrus or cucumber extract feed supplementation did not show any effect on the breast muscle protein FSR of immunologically challenged broilers. The present study has identified potential predictive markers of breast muscle growth and provided new information on broiler chicken breast muscle protein turnover which could be essential for improving the efficiency of broiler chicken meat production.
HostingRepositoryPRIDE
AnnounceDate2024-04-04
AnnouncementXMLSubmission_2024-04-04_04:22:18.728.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEduardo Zarzuela
SpeciesList scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-03 08:05:51ID requested
12024-04-04 04:22:19announced
22024-10-22 06:35:11announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.jprot.2024.105158;
Peinado-Izaguerri J, Corbishley A, Zarzuela E, Pina-Beltr, á, n B, Riva F, McKeegan DEF, Bain M, Mu, ñ, oz J, Bhide M, McLaughlin M, Preston T, Effect of an immune challenge and two feed supplements on broiler chicken individual breast muscle protein synthesis rate. J Proteomics, 299():105158(2024) [pubmed]
Keyword List
submitter keyword: chicken, citrus, FSR, muscle
Contact List
Javier Muñoz
contact affiliationCNIO
contact emailjavier.munozperalta@osakidetza.eus
lab head
Eduardo Zarzuela
contact affiliationCentro Nacional de Investigaciones Oncológicas
contact emaileduzarfer@gmail.com
dataset submitter
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Dataset FTP location
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