PXD044319 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Silencing of the PHLDA1 leads to global proteome changes and differentiation pathways of human neuroblastoma cells |
Description | Neuroblastoma (NB) is the most common extracranial pediatric solid tumor originating from the abnormal development of cells of the sympathoadrenal lineage of the neural crest. Targeting GD2 ganglioside (GD2), a glycolipid expressed on neuroblastoma cells, with GD2 ganglioside-recognizing antibodies affects several pivotal signaling routes that drive or influence the malignant phenotype of the cells. Previously performed gene expression profiling helped us to identify the PHLDA1 (pleckstrin homology-like domain family A member 1) gene as the most upregulated gene in the IMR-32 human neuroblastoma cells treated with the mouse 14G2a monoclonal antibody. Mass spectrometry-based proteomic analyses were applied to better characterize a role of PHLDA1 protein in the response of neuroblastoma cells to chimeric ch14.18/CHO antibody. Additionally, global protein expression profile analysis in the IMR-32 cell line with PHLDA1 silencing revealed increase in biological functions of mitochondria, accompanied by differentiation-like phenotype of the cells. Moreover, mass spectrometry analysis of the proteins co-immunoprecipitated using anti-PHLDA1-specific antibody, selected a group of possible PHLDA1 binding partners. Also, a more detailed analysis suggested that PHLDA1 interacts with the DCAF7/AUTS2 complex, a key component of neuronal differentiation in vitro. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-15 |
AnnouncementXML | Submission_2024-02-15_07:37:14.676.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD044319 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Agata Malinowska |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitinylated lysine; methylthiolated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480; Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-03 06:53:27 | ID requested | |
⏵ 1 | 2024-02-15 07:37:15 | announced | |
2 | 2024-10-22 06:30:01 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: human neuroblastoma cells, GD2 ganglioside-recognizing antibody, mass spectrometry, AUTS2,PHLDA1 silencing |
Contact List
Hanna Rokita |
contact affiliation | Laboratory of Molecular Genetics and Virology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland |
contact email | hanna.rokita@uj.edu.pl |
lab head | |
Agata Malinowska |
contact affiliation | IBB PAS |
contact email | esme@ibb.waw.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044319
- Label: PRIDE project
- Name: Silencing of the PHLDA1 leads to global proteome changes and differentiation pathways of human neuroblastoma cells