PXD044313-1
PXD044313 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative proteomic profiling of HCC1954 cells treated with a CDK12 degrader |
| Description | HCC1954 cells growing at 80% confluency in a 10-cm dish were treated with (R)-2-(4-(9-ethyl-6-(((6-(furan-3-yl)pyridin-3-yl)methyl)amino)-9H-purin-2-yl)morpholin-3-yl)ethan-1-ol (a CDK12 degrader) at 300 nM (0.5% in DMSO) or DMSO as a control for 2 hours. Each was carried out in triplicates. The cells were disrupted in a lysis buffer containing 2% SDS, 2 mM MgCl2, 50 mM triethylammonium bicarbonate, 1x HALT phosphatase and protease inhibitors (ThermoFisher Scientific, Waltham, MA) using Bioruptor Sonicator (Diagenode, Denville, NJ). Proteins were recovered and proteolyzed with trypsin on S-trap columns (PROTIFI, Fairport, NY). The resultant peptides were labeled with 6-plex TMT isobaric reagents (triplicates for 2 conditions), mixed altogether, subfractionated by basic reversed phase high performance liquid chromatography, and analyzed by LC-MS/MS (ThermoFisher, Q-Exactive). The LC-MS/MS data were processed by using Proteome Discoverer v2.4. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-08-02 |
| AnnouncementXML | Submission_2023-08-02_23:38:47.928.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Narae Park |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | TMT6plex; Acetyl; Oxidation; Carbamidomethyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-08-02 23:22:40 | ID requested | |
| ⏵ 1 | 2023-08-02 23:38:48 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: LC-MS/MS based proteomics, tandem mass tag, a CDK12 degrader |
Contact List
| Cheolju lee | |
|---|---|
| contact affiliation | Korea Institute of Science and Technology |
| contact email | clee270@kist.re.kr |
| lab head | |
| Narae Park | |
| contact affiliation | KIST |
| contact email | nr_park@kist.re.kr |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092579/ |
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