PXD044309-1

PXD044309 is an original dataset announced via ProteomeXchange.

Title	The microenvironment dictates glycocalyx construction and immune surveillance
Description	Efforts to identify anti-cancer therapeutics are attempted with in vitro models that do not match the microenvironmental characteristics of human tissues. Using biocompatible hydrogels that mimic healthy or cancerous tissues and conventional or physiologically relevant culture medium, we systematically demonstrate that the chemical and physical properties of the microenvironment regulate the composition and topology of the glycocalyx, a pericellular matrix composed of carbohydrate-conjugates. Remarkably, we find that cancer and age-related changes in the physical properties of the microenvironment are sufficient to adjust immune surveillance via the topology of the glycocalyx, a previously unknown phenomenon observable only with physiologically relevant culture medium.
HostingRepository	PRIDE
AnnounceDate	2026-04-10
AnnouncementXML	Submission_2026-04-10_15:55:30.340.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alicia Richards
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-02 21:28:02	ID requested
⏵ 1	2026-04-10 15:55:30	announced

Dataset with its publication pending

submitter keyword: ECM, metabolism, glycosylation,LC-MS/MS, mechanopharmacology

Danielle L Swaney
contact affiliation	Quantitative Biosciences Institute (QBI), J. David Gladstone Institutes, Department of Cellular and Molecular Pharmacology, University of California, San Francisco
contact email	Danielle.Swaney@ucsf.edu
lab head
Alicia Richards
contact affiliation	Krogan lab
contact email	alicia.richards@ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD044309

PRIDE project URI

• PRIDE
  1. PXD044309
     1. Label: PRIDE project
     2. Name: The microenvironment dictates glycocalyx construction and immune surveillance