PXD044268 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Assay for characterizing adsorption-properties of surfaces (APS) |
Description | Analytes, from sample preparation, until entering an analytical instrument, are prone to adsorb to surfaces, driven by the chemical properties of the surface and the liquids they are dissolved in. This problem can be addressed with internal standards when a single or few known analytes are quantified that are usually not available in omics. However, minimal to no loss of analytes is the aim. Here, we present a novel assay for qualifying and quantifying interactions responsible for adsorption of molecules to surfaces (APS) by using LC-MS/MS-based differential quantitative analysis. To reflect a broad range of chemical interactions with surfaces, a reference mixture of thousands of tryptic peptides, with known compositions was selected, representing a variety of different chemical characteristics. The assay was tested by investigating the adsorption properties of several different vials with different surface chemistries. A significant number of hydrophobic peptides adsorbed to conventional polypropylene vials. In contrast, only few peptides adsorbed to polypropylene vials, assigned as low-protein-binding. The highest number of peptides adsorbed to glass vials driven by electrostatic interactions. In summary, the new assay is suitable to characterize adsorption properties of different surfaces and to approximate the loss of analytes during sample preparation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-30 |
AnnouncementXML | Submission_2024-08-30_00:36:24.616.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bente Siebels |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-01 10:51:31 | ID requested | |
⏵ 1 | 2024-08-30 00:36:25 | announced | |
2 | 2024-10-22 06:57:03 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: adsorption, sample loss, surfaces, label-free quantification, LC-MS/MS |
Contact List
Hartmut Schlueter |
contact affiliation | Section Mass Spectrometric Proteomics, University Medical Center Hamburg-Eppendorf, Martinistraße 52, 20246 Hamburg, Germany |
contact email | h.schlueter@uke.de |
lab head | |
Bente Siebels |
contact affiliation | Section for Mass spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf |
contact email | b.siebels@uke.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044268
- Label: PRIDE project
- Name: Assay for characterizing adsorption-properties of surfaces (APS)