PXD044263-1

PXD044263 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic identification of Rab GTPase interactors in Arabidopsis
Description	Rab GTPases are key spatio-temporal regulators of endomembrane trafficking and endomembrane compartment identity across eukaryotes. This therefore makes them ideal targets for study of endomembrane compartment and trafficking pathway composition. In plants, few definitive interactors of Rab GTPases and cargoes of their associated trafficking pathways are known, hindering our understanding of how plants may accomplish and support some unique aspects and requirements of their post-Golgi trafficking systems. The purpose of this work was to identify candidate interactors of different post-Golgi Rab GTPases in Arabidopsis. Co-IPs were performed against tagged-versions of three different post-Golgi Rab GTPases: RAB-A5c, RAB-A2a and RAB-G3f, as well as against control wild-type (Col-0) plants. Comparative ranking of the different proteomes produced candidate interactor lists enriched in possible specific interactors of each Rab GTPase, as well as generic shared interactors. This dataset forms the basis of the characterisation work of several upcoming publications.
HostingRepository	PRIDE
AnnounceDate	2023-09-21
AnnouncementXML	Submission_2023-09-21_09:01:41.938.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	LiamElliott
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-01 06:34:07	ID requested
⏵ 1	2023-09-21 09:01:42	announced
2	2023-11-14 07:16:27	announced	2023-11-14: Updated project metadata.

submitter keyword: Arabidopsis, Rab GTPase

CharlotteKirchhelle
contact affiliation	Laboratoire Reproduction et DÉveloppement des Plantes, ENS de Lyon
contact email	charlotte.kirchhelle@ens-lyon.fr
lab head
LiamElliott
contact affiliation	RDP lab, ENS de Lyon
contact email	liam.elliott@ens-lyon.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD044263

PRIDE project URI

• PRIDE
  1. PXD044263
     1. Label: PRIDE project
     2. Name: Comparative proteomic identification of Rab GTPase interactors in Arabidopsis