PXD044256 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Plasmodium falciparum MORC protein modulates gene expression through interaction with heterochromatin |
Description | Dynamic control of gene expression is critical for blood stage development of malaria parasites. Here, we used multi-omic analyses to investigate transcriptional regulation by the chromatin-associated microrchidia protein, MORC, during asexual blood stage development of the human malaria parasite Plasmodium falciparum. PfMORC (PF3D7_1468100) interacts with a suite of nuclear proteins, including APETALA2 (AP2) transcription factors (PfAP2-G5, PfAP2-O5, PfAP2-I, PF3D7_0420300, PF3D7_0613800, PF3D7_1107800 , and PF3D7_1239200), a DNA helicase DS60 (PF3D7_1227100), and other chromatin remodelers (PfCHD1, PfEELM2, and PfISWI). Transcriptomic analysis of PfMORCHA-glmS knockdown parasites revealed 163 differentially expressed genes belonging to hypervariable multigene families, along with upregulation of genes mostly involved in host cell invasion. In vivo genome-wide chromatin occupancy analysis during both trophozoite and schizont stages of development demonstrates that PfMORC is recruited to repressed, multigene families, including the var genes in subtelomeric chromosomal regions. Collectively, we find that PfMORC is found in chromatin complexes that play a role in the epigenetic control of asexual blood stage transcriptional regulation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-14 |
AnnouncementXML | Submission_2024-08-14_10:50:34.060.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Veronica Santiago |
SpeciesList | scientific name: Plasmodium falciparum; NCBI TaxID: 5833; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-01 02:58:32 | ID requested | |
⏵ 1 | 2024-08-14 10:50:34 | announced | |
2 | 2024-10-22 06:55:04 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Plasmodium falciparum, MORC, transcription factors, chromatin, epigenetic |
Contact List
Celia R. S. Garcia |
contact affiliation | Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil |
contact email | celiaregarcia@gmail.com |
lab head | |
Veronica Santiago |
contact affiliation | University of Southampton |
contact email | veronicafeijoli@usp.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044256
- Label: PRIDE project
- Name: Plasmodium falciparum MORC protein modulates gene expression through interaction with heterochromatin