PXD044240 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in C. elegans |
Description | Assembly of the DNA helicase known as CMG (CDC45-MCM-GINS) is the key regulated step during DNA replication initiation in eukaryotes. Using the Caenorhabditis elegans embryo as a model system, we identify a new CMG assembly factor called DNSN-1, which associates with the BRCT-domain protein MUS-101. We show that DNSN-1 is required to recruit the GINS complex to chromatin and find that DNSN-1 positions GINS on the MCM-2-7 helicase motor, by direct binding of DNSN-1 to GINS and MCM-3, on interfaces that are important for initiation and essential for viability. |
HostingRepository | PRIDE |
AnnounceDate | 2023-10-24 |
AnnouncementXML | Submission_2023-10-24_12:30:50.334.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD044240 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | KARIMLABIB |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-31 16:19:08 | ID requested | |
⏵ 1 | 2023-10-24 12:30:51 | announced | |
2 | 2023-11-14 09:09:42 | announced | 2023-11-14: Updated project metadata. |
Publication List
Xia Y, Sonneville R, Jenkyn-Bedford M, Ji L, Alabert C, Hong Y, Yeeles JTP, Labib KPM, . Science, 381(6664):eadi4932(2023) [pubmed] |
10.6019/PXD044240; |
Keyword List
submitter keyword: DONSON,C. elegans, Meier Gorlin Syndrome, MUS-101, DNSN-1, CMG helicase, DNA replication |
Contact List
KarimLabib |
contact affiliation | MRC Protein Phosphorylation & Ubiquitylation Unit, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland - UK |
contact email | kpmlabib@dundee.ac.uk |
lab head | |
KARIMLABIB |
contact affiliation | University of Dundee |
contact email | kpmlabib@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044240
- Label: PRIDE project
- Name: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in C. elegans