PXD044226 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Investigating the effect of fluid flow on glomerular cell-derived matrix |
| Description | The glomerular basement membrane has a complex composition and structure in vivo. In vitro models of the glomerular capillary wall have been developed using microfluidic and perfusion systems, however, how the extracellular matrix alters under static and flow conditions has not yet been investigated. Here we performed proteomic analyses following a previously described workflow (Byron et al., 2014; Rashid et al., 2012; Soteriou et al., 2013) to investigate the protein composition of podocyte and glomerular endothelial coculture cell-derived matrix and whether this was altered when cells experienced shear stress induced by flow. Our results reveal a distinct separation between flow and static-treated samples and highlight compositional changes in the matrix in the microfluidic in vitro model, and confirm the flow impacted the abundance of certain matrix proteins. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-02 |
| AnnouncementXML | Submission_2026-02-02_06:29:36.055.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD044226 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Mychel Morais |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-07-31 07:51:14 | ID requested | |
| ⏵ 1 | 2026-02-02 06:29:36 | announced | |
Publication List
Keyword List
| submitter keyword: Glomerular matrix, Microfluidic bioreactor,Glomerular filtration barrier |
Contact List
| Rachel Lennon |
|---|
| contact affiliation | Director of the Wellcome Centre for Cell-Matrix Research Wellcome Trust Centre for Cell Matrix Research School of Biological Sciences Faculty of Biology, Medical and Health Sciences University of Manchester |
| contact email | rachel.lennon@manchester.ac.uk |
| lab head | |
| Mychel Morais |
|---|
| contact affiliation | The University of Manchester |
| contact email | mychel.morais@manchester.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD044226 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044226
- Label: PRIDE project
- Name: Investigating the effect of fluid flow on glomerular cell-derived matrix
