PXD044191-1

PXD044191 is an original dataset announced via ProteomeXchange.

Title	Harnessing the acceptor substrate promiscuity of Clostridium botulinum Maf glycosyltransferase to glyco-engineer mini-flagellin protein chimeras
Description	In this study, we identified the O-glycosylation sites in deletion constructs of recombinant Clostridium botulinum flagellin (CbFla) expressed from the E. coli strain, EV136 (K1:K12 strains engineered by Prof. Eric Vimr’s group to accumulate CMP-sialic acid in the cytosol). The recombinant flagellins were expressed with or without co-expression of motility associated factor (Maf) (flagellin nonulosonic acid glycosyltransferase) in these strains, purified and subjected to MS/MS analysis at Taplin Biological Mass Spectrometry facility. We also studied the O-glycosylation of recombinant Clostridium botulinum flagellin (CbFla) co-expressed with Geobacillus kaustophilus Maf. We also studied the O-glycosylation of flagellin chimeras made by grafting mini-flagellin sequences on to an unrelated protein with an F-type lectin domain from Streptosporangium roseum SrNaFLD.
HostingRepository	PRIDE
AnnounceDate	2024-08-22
AnnouncementXML	Submission_2024-08-22_06:14:15.126.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ramya Chakravarthy
SpeciesList	scientific name: Clostridium botulinum str. LANGELAN F; NCBI TaxID: 696044;
ModificationList	O-glycosylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-28 11:35:05	ID requested
⏵ 1	2024-08-22 06:14:15	announced
2	2024-10-22 06:55:40	announced	2024-10-22: Updated project metadata.

10.1038/S42003-024-06736-Y;

submitter keyword: LTQ Orbitrap Velos Pro ion-trap mass spectrometer, nonulosonic acid, O-glycosylation,Neu5Ac, sialic acid, KDO, Clostridium botulinum, Gram positive, flagellin, Geobacillus kaustophilus, Maf

Ramya Chakravarthy
contact affiliation	CSIR- Institute of Microbial Technology, Chandigarh
contact email	ramya@imtech.res.in
lab head
Ramya Chakravarthy
contact affiliation	CSIR-Institute of Microbial Technology, Chandigarh
contact email	ramya@imtech.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD044191

PRIDE project URI

• PRIDE
  1. PXD044191
     1. Label: PRIDE project
     2. Name: Harnessing the acceptor substrate promiscuity of Clostridium botulinum Maf glycosyltransferase to glyco-engineer mini-flagellin protein chimeras