PXD044190-2

PXD044190 is an original dataset announced via ProteomeXchange.

Title	A phylogenetic approach to explore the Aspergillus fumigatus conidial surfome and its role in pathogenesis
Description	Aspergillus fumigatus is the most important pulmonary fungal pathogen and is able to cause several diseases collectively called aspergillosis. Conidia is the most important infection structure making the initial contact with the human host. Here, we used a phylogenomic approach comparing proteins present in the A. fumigatus conidial surface, two closely related non-pathogenic species A. fischeri and A. oerlinghausenensis, and the far-related pathogenic A. lentulus. We were able to identify 62 proteins specifically expressed on A. fumigatus conidial surface. We deleted 42 of the encoding-genes and observed that many of them have altered susceptibility to macrophage killing, penetration and damage to epithelial cells, and cytokine production. We demonstrated that one of these genes encoding a glycosylasparaginase is modulating IL-1β levels and is important for the infection in an immunocompetent murine model. Our results provide opportunities for characterizing A. fumigatus effectors important for evasion and modulation of the immune response.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:47:31.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giuseppe Palmisano
SpeciesList	scientific name: Fungi; NCBI TaxID: NCBITaxon:4751; scientific name: Neosartorya fumigata (Aspergillus fumigatus); NCBI TaxID: 746128; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-28 11:03:08	ID requested
1	2024-06-26 13:02:30	announced
⏵ 2	2024-10-22 06:47:32	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: conidia, glycosylasparaginase, macrophages,Aspergillus fumigatus

Giuseppe Palmisano
contact affiliation	University of Sao Paulo
contact email	palmisano.gp@gmail.com
lab head
Giuseppe Palmisano
contact affiliation	University of Sao Paulo
contact email	palmisano.gp@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD044190

PRIDE project URI

• PRIDE
  1. PXD044190
     1. Label: PRIDE project
     2. Name: A phylogenetic approach to explore the Aspergillus fumigatus conidial surfome and its role in pathogenesis