PXD044165-2

PXD044165 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry identification of interacting proteins of the NP protein from genotype IV NDV Herts/33 and the NP protein from genotype VII NDV strain I4
Description	Newcastle Disease Virus (NDV) is one of the most threatening viruses to the poultry industry, and it also exhibits oncolytic properties. In our research, we identified a non-oncolytic strain, genotype VII NDV strain I4, where the lack of oncolytic ability is attributed to its NP protein. To explore the mechanism of NP-mediated oncolysis by NDV, we focused on the differential interacting proteins of the NP proteins from two strains: the highly oncolytic Herts/33 strain and the poorly oncolytic I4 strain. The mass spectrometry results show the interacting protein profiles of the NP proteins from both virus strains. After infecting HeLa cells with each strain, we conducted immunoprecipitation using antibodies against the NP protein. Due to the lower expression of I4-associated proteins during replication, we further performed immunoprecipitation experiments using separately overexpressed NP proteins from I4 and Herts/33.
HostingRepository	iProX
AnnounceDate	2023-07-28
AnnouncementXML	Submission_2024-03-07_19:16:15.776.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiufan Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-27 23:12:15	ID requested
1	2023-07-27 23:12:39	announced
⏵ 2	2024-03-07 19:16:17	announced	2024-03-08: Update information.

Liao T, Chen Y, Guo L, Zhu S, Zhan T, Lu X, Xu H, Hu Z, Hu J, Gu M, Liu X, Wang X, Hu S, Liu X, The NP protein of Newcastle disease virus dictates its oncolytic activity by regulating viral mRNA translation efficiency. PLoS Pathog, 20(2):e1012027(2024) [pubmed]

submitter keyword: mass spectrometry, Newcastle disease virus, NP protein, oncolytic virus

Xiufan Liu
contact affiliation	Yangzhou University
contact email	xfliu@yzu.edu.cn
lab head
Xiufan Liu
contact affiliation	Yangzhou University
contact email	2693040819@qq.com
dataset submitter

iProX dataset URI