PXD044162-1

PXD044162 is an original dataset announced via ProteomeXchange.

Title	Systematic affinity purification mass spectrometry (AP-MS) reveals LCMV interacted human proteins
Description	To systematically map the arenavirus‒host protein interaction, we employed a systematic affinity purification mass spectrometry (AP-MS) approach to define the human proteins that physically interact with LCMV proteins. The four LCMV proteins (NP, LP, GPC, Z) and control protein GFP were tandemly cloned with Twin-Strep tags at C-terminal based on the same backbone pCAGGs vector respectively. Proteins were expressed in HEK293T cells followed by affinity-purification based on Strep-Tactin System. We summarized the number of human proteins interacting with each indicated LCMV protein baits and identified 284 human proteins with high-confidence PPIs (log2 enriched fold > 1, P < 0.05).
HostingRepository	iProX
AnnounceDate	2023-07-28
AnnouncementXML	Submission_2023-07-27_23:07:36.372.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leike Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-27 23:05:16	ID requested
⏵ 1	2023-07-27 23:07:36	announced

Dataset with its publication pending

submitter keyword: LCMV, AP-MS, Protein Interaction

Leike Zhang
contact affiliation	Wuhan Institute of Virology, Chinese Academy of Sciences
contact email	zhangleike@wh.iov.cn
lab head
Leike Zhang
contact affiliation	Wuhan Institute of Virology, Chinese Academy of Sciences
contact email	zhangleike@wh.iov.cn
dataset submitter

iProX dataset URI