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PXD044152-1

PXD044152 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEffect of diet and association with fertility outcomes in semen proteome and transcriptome from wild and captive Black-footed ferrets
DescriptionThe black-footed ferret (Mustela nigripes) is a star example of the efforts of conservation programs in bringing endangered species back from the brink of extinction. As one of the world’s most endangered mammals, the vast majority of black-footed ferrets living in the wild today are the offspring of a founding captive population. The success of this ongoing breeding program, however, is threatened by inbreeding depression and the observed decline in pregnancy rates since its founding. As the wild and modern captive populations share a genetic history, the greatest difference between the two groups is the captive environment of the breeding program. In this study, we used RNA sequencing and proteomics for the first time in black-footed ferrets to explore whether the diet of wild ferrets versus captive diet variants could explain the differences in fertility and sperm characteristics observed between each population. We find that changes in both the transcriptional and proteomic profile of black-footed ferret ejaculate are strongly associated with differences in fertility, especially in pathways associated with innate immunity and metabolism; that transcriptional changes are further exacerbated by diet. Overall, our results support the hypothesis of ongoing environmental-dependent inbreeding depression in the black-footed ferret, with a need to re-evaluate dietary and environmental parameters of the conservation program; and also illustrates the value of multi-level genomics for conservation management programs.
HostingRepositoryPRIDE
AnnounceDate2024-10-08
AnnouncementXMLSubmission_2024-10-08_11:39:04.289.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJuan Vazquez
SpeciesList scientific name: Mustela nigripes; NCBI TaxID: 77151;
ModificationListphosphorylated residue
Instrument6220 Time-of-Flight LC/MS; MALDI Synapt MS; Synapt MS; Q Exactive HF; LCQ Classic; TripleTOF 5600; 6410 Triple Quadrupole LC/MS; Q Exactive; LTQ Orbitrap; 4800 Proteomics Analyzer; 6340 Ion Trap LC/MS; Orbitrap Fusion Lumos; LTQ; Orbitrap Exploris 480; LTQ Orbitrap Velos; Q TRAP; Q-Tof Ultima; maXis; LTQ FT; autoflex; ultraflex; QSTAR; 4700 Proteomics Analyzer; Orbitrap Fusion; 6520A Quadrupole Time-of-Flight LC/MS; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-27 11:38:29ID requested
12024-10-08 11:39:04announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: black-footed ferret, fertility,conservation, diet, genomics
Contact List
Juan Manuel Vazquez
contact affiliationDepartment of Integrative Biology, University of California Berkeley
contact emailaging@berkeley.edu
lab head
Juan Vazquez
contact affiliationDepartment of Integrative Biology University of California, Berkeley
contact emailaging@berkeley.edu
dataset submitter
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