PXD044135-2

PXD044135 is an original dataset announced via ProteomeXchange.

Title	Quantitative differences in rumen epithelium proteins in lambs fed wheat, perennial wheat or perennial wheat plus lucerne.
Description	The rumen is a major the site of digestion and absorption of energy predominantly in the form of short chain fatty acids as well as amino acids and simple carbohydrates. The value of crops such as perennial wheat (PW) for grain and grazing compared to conventional wheat (W), or the addition of lucerne to PW (PWL) is still being determined. The research sought to determine if these diets representing high potassium (K): low sodium (Na) ratio (W, PW and PWL) was associated with changes in the membrane bound proteins that transport nutrients in the rumen epithelium (RE). Information about these proteins and the influence of different diets is scarce due to difficulties developing a procedure to examine them using quantitative proteomics. The research focuses on proteins extracted using an enzymatic procedure (Bond et al. 2019) from the rumen epithelium layer of the sheep’s’ forestomach. A membrane protein enriched fraction was then extracted and prepared for TMT-MS. The comprehensive assignment of proteins (>4000) to cellular location and quantification of differences in protein abundance will progress the annotation and understanding of protein functions in the ruminant genomes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:30.688.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karthik Shantharam kamath
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-27 04:40:08	ID requested
1	2023-10-24 08:47:50	announced
⏵ 2	2023-11-14 09:06:31	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:07:39	announced	2024-10-22: Updated project metadata.

Bond JJ, Refshauge G, Newell MT, Holman BWB, Wheeler D, Woodgate S, Kamath KS, Hayes RC, Quantitative Differences in Rumen Epithelium Proteins in Lambs Fed Wheat, Perennial Wheat, or Perennial Wheat plus Lucerne. Proteomes, 11(3):(2023) [pubmed]

submitter keyword: epithelium,nutrient, transport, TMT-MS, rumen, proteome, membrane

Dr Jude Bond
contact affiliation	NSW Department of Primary Industries, JSF Barker Building, Trevenna road, University of New England, Armidale, NSW 2351
contact email	jude.bond@dpi.nsw.gov.au
lab head
Karthik Shantharam kamath
contact affiliation	Macquarie university
contact email	karthikskamath@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044135
     1. Label: PRIDE project
     2. Name: Quantitative differences in rumen epithelium proteins in lambs fed wheat, perennial wheat or perennial wheat plus lucerne.