PXD044127-1

PXD044127 is an original dataset announced via ProteomeXchange.

Title	Kidney tissue pipeline for multi-omics and histological analysis - LCMSMS of porcine (pig) kidney biopsies
Description	In this study, our primary objective was to establish a streamlined workflow for the collection of kidney biopsies obtained during clinical procedures. Our aim was to ensure that this workflow integrates into the daily routines while also preserving the integrity of the precious samples for subsequent analysis using advanced techniques. To achieve this, we modified a robust protocol for dissociating single nuclei from variously stored kidney tissue samples, including snap frozen, RNAlater, and CellCover preserved specimens. These single nucleus (sn) suspensions were then processed using the widely recognized Chromium 10X Genomics platform. We conducted snRNAseq analysis on the resulting datasets from each storage method to identify any potential variations in transcriptomic profiles. Furthermore, we assessed the suitability of the preservation media for additional analyses such as proteomics, metabolomics, and the preservation of tissue architecture for immunofluorescence staining. This evaluation aimed to determine if the chosen preservation methods effectively maintained the necessary quality and characteristics of the samples for diverse analytical approaches. Overall, our study aimed to develop an efficient and adaptable workflow for the collection and preservation of clinically obtained kidney biopsies, enabling comprehensive analysis using cutting-edge techniques while ensuring the preservation of valuable samples.
HostingRepository	PRIDE
AnnounceDate	2025-06-27
AnnouncementXML	Submission_2025-06-27_03:15:27.939.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz Lassé
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-27 01:52:06	ID requested
⏵ 1	2025-06-27 03:15:28	announced

10.1152/AJPRENAL.00404.2023;

submitter keyword: kidney, proteomics, cryo-preservation, biobank, pig

Markus M. Rinschen
contact affiliation	III. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
contact email	m.rinschen@uke.de
lab head
Moritz Lassé
contact affiliation	Zentrum für Innere Medizin, III. Medizinische Klinik und Poliklinik (Nephrologie/Rheumatologie/Endokrinologie), Universitätsklinikum Hamburg-Eppendorf (UKE), Hamburg, Germany
contact email	moritz.lasse@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD044127

PRIDE project URI

• PRIDE
  1. PXD044127
     1. Label: PRIDE project
     2. Name: Kidney tissue pipeline for multi-omics and histological analysis - LCMSMS of porcine (pig) kidney biopsies