PXD044113-1

PXD044113 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The maintenance of oocytes in the mammalian ovary involves extreme protein longevity
Description	In mammals, oocytes are formed in the female embryo and need to be preserved in the ovary to ensure the viability of the next generation. How oocytes are maintained for decades is unclear. Here, we combined pulse-chase stable isotope labelling coupled with mass spectrometry, single-cell RNA-seq, microscopy and NanoSims to create an atlas of protein homeostasis in mouse oocytes and ovaries over the entire reproductive lifespan. Our results show that protein turnover in the ovary is much slower than in other organs, with hundreds of extremely long-lived proteins across a broad range of complexes and pathways, including mitochondria, ribosomes and the cytoskeleton. We propose that slow protein turnover helps to maintain proteostasis in oocytes and the ovary over long periods, protecting the germline across generations.
HostingRepository	MassIVE
AnnounceDate	2024-04-05
AnnouncementXML	Submission_2024-04-05_12:03:12.011.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luisa Welp
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Label:13C(6)
Instrument	Orbitrap Exploris 480; Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-26 10:52:09	ID requested
⏵ 1	2024-04-05 12:03:12	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: oocytes, long-lived proteins, SILAC

submitter keyword: oocytes, long-lived proteins, SILAC

Contact List

Dr. Melina Schuh
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	melina.schuh@mpinat.mpg.de
lab head
Dr. Juliane Liepe
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	juliane.liepe@mpinat.mpg.de
lab head
Prof. Henning Urlaub
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	henning.urlaub@mpinat.mpg.de
lab head
Luisa Welp
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	luisa.welp@mpinat.mpg.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000092528/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000092528/