PXD044110 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | PIAS2b dsRNAi repression kills by mitotic catastrophe anaplastic thyroid and non-thyroid carcinomas, but not normal thyroid or differentiated carcinomas |
| Description | The E3 SUMO ligase PIAS2 is expressed at high levels in differentiated papillary thyroid carcinomas but at low levels in anaplastic thyroid carcinomas (ATC), an undifferentiated cancer with very high mortality. Double-stranded RNA–directed RNA interference (dsRNAi) targeting the PIAS2 isoform beta (PIAS2b) inhibits growth of ATC cell lines and patient primary cultures in vitro and orthotopic patient-derived xenografts (oPDX) in vivo, but not of thyroid cell lines or non-anaplastic primary thyroid cultures (differentiated carcinoma, benign lesions, or normal). PIAS2b-dsRNAi also has an anti-cancer effect on other anaplastic human cancers (pancreas, lung, and gastric).
Mechanistically, PIAS2b is required for proper mitotic spindle and centrosome assembly, and it is a dosage-sensitive protein in ATC. Strikingly, PIAS2b-dsRNAi induces mitotic catastrophe at prophase. High-throughput proteomics revealed the proteasome (PSMC5) and spindle cytoskeleton as direct targets of PIAS2b SUMOylation at mitotic initiation. PIAS2b-dsRNAi is a promising therapy for ATC and other aggressive anaplastic cancers.
|
| HostingRepository | PRIDE |
| AnnounceDate | 2024-05-20 |
| AnnouncementXML | Submission_2024-05-20_01:31:15.071.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Susana Bravo |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | sumoylated lysine |
| Instrument | TripleTOF 6600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-07-26 09:58:28 | ID requested | |
| 1 | 2024-04-08 11:13:20 | announced | |
| ⏵ 2 | 2024-05-20 01:31:16 | announced | 2024-05-20: Updated project metadata. |
Publication List
| Rodrigues JS, Chenlo M, Bravo SB, Perez-Romero S, Suarez-Fari, ñ, a M, Sobrino T, Sanz-Pamplona R, Gonz, á, lez-Prieto R, Blanco Freire MN, Nogueiras R, L, ó, pez M, Fugazzola L, Cameselle-Teijeiro JM, Alvarez CV, dsRNAi-mediated silencing of PIAS2beta specifically kills anaplastic carcinomas by mitotic catastrophe. Nat Commun, 15(1):3736(2024) [pubmed] |
| 10.1038/s41467-024-47751-1; |
Keyword List
| submitter keyword: SUMO-affinity columna, PIAS2,Anaplastic Thyroid Cancer, immunoprecipitation, E3 SUMO ligase |
Contact List
| Susana Belén Bravo López |
|---|
| contact affiliation | Instituto de Investigaciones Sanitarias de Santiago de Compostela |
| contact email | sbbravo@gmail.com |
| lab head | |
| Susana Bravo |
|---|
| contact affiliation | FIDIS |
| contact email | sbbravo@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD044110 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044110
- Label: PRIDE project
- Name: PIAS2b dsRNAi repression kills by mitotic catastrophe anaplastic thyroid and non-thyroid carcinomas, but not normal thyroid or differentiated carcinomas
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