PXD044103-1

PXD044103 is an original dataset announced via ProteomeXchange.

Title	Conformational dynamics underlying Atypical Chemokine Receptor 3 activation
Description	Here we used Hydrogen/Deuterium exchange mass spectrometry (HDX-MS) to examine the binding mode and mechanism of action of various small-molecule ACKR3 ligands of different efficacy for β-arrestin recruitment. Our results show that activation or inhibition of ACKR3 is largely governed by intracellular conformational changes of helix 6, intracellular loop 2 and helix 7, while the DRY motif becomes protected during both processes. Moreover, HDX-MS identifies the binding sites and the allosteric modulation of ACKR3 upon β-arrestin 1 binding.
HostingRepository	PRIDE
AnnounceDate	2024-07-16
AnnouncementXML	Submission_2024-07-16_08:30:13.057.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Functional Proteomics Platform FPP
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	nanoACQUITY UPLC with HDX Technology

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-26 06:57:51	ID requested
⏵ 1	2024-07-16 08:30:13	announced
2	2024-10-22 06:50:28	announced	2024-10-22: Updated project metadata.

10.1073/pnas.2404000121;

Otun O, Aljamous C, Del Nero E, Arimont-Segura M, Bosma R, Zarzycka B, Girbau T, Leyrat C, de Graaf C, Leurs R, Durroux T, Granier S, Cong X, Bechara C, Conformational dynamics underlying atypical chemokine receptor 3 activation. Proc Natl Acad Sci U S A, 121(30):e2404000121(2024) [pubmed]

submitter keyword: MD,GPCR, HDX-MS

Cherine Bechara
contact affiliation	University of Montpellier, Institut de Génomique Fonctionnelle, France
contact email	Cherine.bechara@igf.cnrs.fr
lab head
Functional Proteomics Platform FPP
contact affiliation	FPP CNRS
contact email	contact@fpp.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD044103

PRIDE project URI

• PRIDE
  1. PXD044103
     1. Label: PRIDE project
     2. Name: Conformational dynamics underlying Atypical Chemokine Receptor 3 activation