PXD044094-1

PXD044094 is an original dataset announced via ProteomeXchange.

Title	An axonemal intron splicing program sustains Plasmodium male development
Description	To gain further insights into the post-transcriptional regulation process in the male sexual stage of Plasmodium, we focused on RBPm1, the most specifically expressed RNA-binding protein in the male gametocyte of P. yoelii, for in-depth investigation. In the first part of this study, we identified RBPm1 as a splicing activator, facilitating the splicing of 30 introns among 26 genes in the male gametocytes. Next, we sought to elucidate how RBPm1 exerts its splicing-promoting function. To achieve this, we performed biotin-based proximity labeling experiments on RBPm1, identifying 113 proteins that interact with RBPm1. Among these proteins, all 13 subunits of the spliceosome E complex were present, while other components of the splicing cycle were not significantly enriched. This suggests that RBPm1 participates in the initiation step of RNA splicing by interacting with the E-complex to promote intron splicing.
HostingRepository	iProX
AnnounceDate	2023-07-26
AnnouncementXML	Submission_2023-08-23_19:48:40.324.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peijia Wu
SpeciesList	scientific name: Plasmodium yoelii yoelii 17XNL; NCBI TaxID: 352914;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-25 21:03:32	ID requested
⏵ 1	2023-08-23 19:48:40	announced

Dataset with its publication pending

submitter keyword: RNA binding protein, spliceosome, malaria

Jing Yuan
contact affiliation	Xiamen University
contact email	yuanjing@xmu.edu.cn
lab head
Peijia Wu
contact affiliation	Xiamen University
contact email	21620220156999@stu.xmu.edu.cn
dataset submitter

iProX dataset URI