PXD044090-1
PXD044090 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Lactylome analysis reveals HADHA lactylation controls energy homeostasis to promote sepsis-induced myocardial depression. |
Description | Serum lactate levels are interpreted as a marker of tissue hypoxia and often used clinically as an indicator of severity and outcome of patients with sepsis/septic shock. Glycolysis-derived lactate was identified as a substrate for lysine lactylation (Kla), which is a recently described type of posttranslational modification. However, the impact of lactylation on biological processes of sepsis-induced myocardial depression remains largely unknown. Here, we conduct lactylome and proteome profiling, and quantify 1,127 Kla sites, of which 83 are differentially lactated in sepsis-induced myocardial depression. We develop a deep learning-based framework to prioritize the lactylation at K166 and K728 of trifunctional enzyme subunit alpha (HADHA) to be functionally important. We perform validations to show that lactylation of HADHA at K166 and K728 is influenced both by endogenous and exogenous lactate, and further verify that lactylation at K166 and K728 inhibits the activity of HADHA, decreasing ATP production, an |
HostingRepository | iProX |
AnnounceDate | 2023-07-26 |
AnnouncementXML | Submission_2023-07-25_19:45:23.784.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tiening Zhang |
SpeciesList | scientific name: Rattus rattus; NCBI TaxID: 10117; |
ModificationList | lactic acid |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-07-25 19:45:05 | ID requested | |
⏵ 1 | 2023-07-25 19:45:24 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Lactylation, metabolism, sepsis, heart dysfunction, lactylome |
Contact List
Tiening Zhang | |
---|---|
contact affiliation | Shengjing Hospital of China Medical University |
contact email | cmuztn@vip.qq.com |
lab head | |
Tiening Zhang | |
contact affiliation | Shengjing Hospital of China Medical University |
contact email | cmuztn@vip.qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |