PXD044043 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Widespread cysteine persulfidation in activated macrophages as a protective mechanism against oxidative-inflammatory stress |
Description | A hallmark of immune-inflammatory responses is the generation of reactive oxygen and nitrogen species by innate immune cells, in particular macrophages. How macrophages cope with excess oxidant production and associated redox stress is not fully understood. Recent evidence implicates reactive sulfur species (RSS) in inflammatory responses, however, how RSS affect macrophage function and its ability to cope with oxidative-inflammatory stress remains poorly understood. Herein, we investigated endogenous pathways of RSS biogenesis and clearance in macrophages, and in particular, explored how hydrogen sulfide (H2S) and thiol persulidation influences macrophage oxidative-inflammatory response. We report that classical activation of mouse or human macrophages with lipopolysaccharide and interferon-γ (LPS/IFN-γ) triggers a marked production of H2S/RSS, leading to a widespread increase in protein persulfidation. Endogenous H2S/persulfidation levels were governed by the activity of the cystine importer xCT, the H2S-generating enzyme cystathionine γ-lyase and the sulfide-metabolizing enzyme sulfide quinone oxidoreductase (SQOR). High levels of H2S/persulfidation, observed upon LPS/IFN-γ stimulation or SQOR inhibition, were associated with a state of increased resistance to oxidative stress. Furthermore, enhanced persulfidation correlated with attenuated activation of the macrophage inflammasome and diminished inflammatory cell death. These findings shed light on the metabolism and effects of RSS in macrophages and point to an important role for persulfides in enabling macrophages to cope with oxidative-inflammatory stress. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-21 |
AnnouncementXML | Submission_2024-05-21_07:06:43.341.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tamar Ziv |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-24 08:49:46 | ID requested | |
⏵ 1 | 2024-05-21 07:06:44 | announced | |
Publication List
10.1016/j.redox.2024.103125; |
Salti T, Braunstein I, Haimovich Y, Ziv T, Benhar M, Widespread S-persulfidation in activated macrophages as a protective mechanism against oxidative-inflammatory stress. Redox Biol, 72():103125(2024) [pubmed] |
Keyword List
submitter keyword: oxidative-inflammatory stress, macrophages |
Contact List
Moran Benhar |
contact affiliation | Department of Biochemistry, Rappaport Faculty of Medicine, Technion–Israel Institute of Technology, Haifa, 31096, Israel |
contact email | benhar@technion.ac.il |
lab head | |
Tamar Ziv |
contact affiliation | Technion |
contact email | tamarz@technion.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044043
- Label: PRIDE project
- Name: Widespread cysteine persulfidation in activated macrophages as a protective mechanism against oxidative-inflammatory stress