PXD044014 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mycobacterium tuberculosis, LC-MS/MS |
| Description | Antibiotic resistance in Mycobacterium tuberculosis (Mtb) is a major public health problem worldwide. Therefore, it is of great significance to study the mutational pathways of susceptive Mtb to drug resistance. Multi-omics approach is ideal to explore more about other molecular mechanisms implicated in drug resistance. In this study, we respectively constructed continuous capreomycin (CAP) and amikacin (AKM)-resistant strain models in vitro using the increasing drug concentration gradients. Based on these models, we use multi-omics techniques and biological experiments to reveal the drug resistance mutations caused by CAP or AKM, and obtain other factors that can cause drug resistance besides the mutations, and explain the differences in cross-resistance pathways caused by AKM and CAP. Our results showed that in addition to Gly232AsptlyA and Trp120fstlyA directly associated with low level resistance to CAP, some mutations, including Ala48ValmmaA2, Gln19ArgrpmA and eis (c.-14c>t), were associated with cross-resistance caused by CAP. The transcriptome-proteome-lipid metabolome analysis of representative induced strains showed that cross-resistance of CAP-induced strains was caused by a combination of many aspects, which was obviously different from the induction of AKM. The results of this study will advance our understanding of the CAP-resistant mechanisms. To a certain extent, it is also conducive to improve the clinical understanding of capreomycin and rational use of CAP. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-15 |
| AnnouncementXML | Submission_2025-12-14_16:12:48.772.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuchuan Zhao |
| SpeciesList | scientific name: Mycobacterium tuberculosis complex sp. AY1MRC; NCBI TaxID: NEWT:2664891; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-07-23 23:22:21 | ID requested | |
| ⏵ 1 | 2025-12-14 16:12:49 | announced | |
Publication List
| 10.1016/j.crmicr.2025.100507; |
| Zhao Y, Zhang C, Wang X, Feng H, Yu M, Chen X, Liang J, Hong J, Huang S, Lin Y, Chen Y, Wei W, . Curr Res Microb Sci, 9():100507(2025) [pubmed] |
Keyword List
| submitter keyword: Mycobacterium tuberculosis |
Contact List
| Yu chuan.Zhao |
|---|
| contact affiliation | Center for Tuberculosis Control of Guangdong Province, Guangzhou, 510630, China |
| contact email | zyc0934130326@163.com |
| lab head | |
| Yuchuan Zhao |
|---|
| contact affiliation | Center for Tuberculosis Control of Guangdong Province, Guangzhou, 510630, China |
| contact email | zyc0934130326@163.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044014
- Label: PRIDE project
- Name: Mycobacterium tuberculosis, LC-MS/MS
