PXD044001-2

PXD044001 is an original dataset announced via ProteomeXchange.

Title	A clinically applicable connectivity signature for glioblastoma includes the tumor network driver CHI3L1
Description	Tumor microtubes (TMs) connect glioma cells to a network with considerable relevance for tumor progression and therapy resistance. The determination of TM-interconnectivity in individual tumors has been challenging and the impact on patient survival unresolved. Here, a connectivity signature from single-cell RNA-sequenced (scRNA-Seq) xenografted primary glioblastoma (GB) cells has been established using a dye uptake methodology, confirmed with recording of cellular calcium epochs and validated with clinical correlations. Astrocyte-like and mesenchymal-like GB cells have the highest connectivity signature scores in scRNA-sequenced patient-derived xenografts and patient samples. In large GB cohorts, network connectivity correlated with the mesenchymal subtype and dismal patient survival. CHI3L1 has been identified and validated as a robust molecular marker of connectivity with functional relevance. The connectivity signature allows novel insights into brain tumor biology, provides a proof-of-principle that tumor cell TM-connectivity is relevant for patients’ prognosis, and serves as a robust prognostic biomarker.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:15:49.540.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tobias Kessker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-22 04:17:05	ID requested
1	2023-11-26 23:52:00	announced
⏵ 2	2024-10-22 06:15:49	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: gene expression signature,glioblastoma, connectivity, tumor microtubes, single-cell RNA-sequencing

Wolfgang Wick
contact affiliation	Heidelberg University Hospital, Department of Neurology
contact email	wolfgang.wick@med.uni-heidelberg.de
lab head
Tobias Kessker
contact affiliation	DKFZ Heidelberg
contact email	t.kessler@dkfz-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD044001

PRIDE project URI

• PRIDE
  1. PXD044001
     1. Label: PRIDE project
     2. Name: A clinically applicable connectivity signature for glioblastoma includes the tumor network driver CHI3L1