PXD043987 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Post-translational modification of the transcription factor SPATULA by SECRET AGENT and SPINDLY promotes Arabidopsis style development. |
Description | O-linked-β-N-Acetylglucosamine (O-GlcNAc) and O-fucose are two sugar-based post-translational modifications (PTMs) whose mechanistic role in plant signalling and transcriptional regulation is still largely unknown. Here, we investigated how two O-glycosyltransferase enzymes of Arabidopsis thaliana, SPINDLY (SPY) and SECRET AGENT (SEC) promote the activity of the bHLH transcription factor SPATULA (SPT), during morphogenesis of the plant female reproductive organ apex, the style. SPY and SEC modify N-terminal residues of SPT in vivo and in vitro by attaching O-fucose and O-GlcNAc, respectively. This post-translational regulation does not impact SPT homo- and hetero-dimerisation events, although it enhances the affinity of SPT for the kinase PINOID (PID) gene locus and its transcriptional repression. Our findings offer the first mechanistic example of the effect of O-GlcNAc and O-fucose on the activity of a plant transcription factor and reveal a previously unrecognized roles for SEC and SPY in orchestrating style elongation and shape. |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-22 |
AnnouncementXML | Submission_2024-01-22_09:36:02.169.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD043987 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Gerhard Saalbach |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | deoxyhexosylated residue; N-acetylaminohexosylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-21 08:42:19 | ID requested | |
⏵ 1 | 2024-01-22 09:36:02 | announced | |
Publication List
Keyword List
submitter keyword: SPY, mass spectrometry, SPT,O-glycosylation, gynoecium, SEC |
Contact List
Laila Moubayidin |
contact affiliation | Group leader Cell&Developmental Biology John Innes Centre Norwich, UK |
contact email | Laila.Moubayidin@jic.ac.uk |
lab head | |
Gerhard Saalbach |
contact affiliation | Biological Chemistry |
contact email | gerhard.saalbach@jic.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043987
- Label: PRIDE project
- Name: Post-translational modification of the transcription factor SPATULA by SECRET AGENT and SPINDLY promotes Arabidopsis style development.