PXD043965-3

PXD043965 is an original dataset announced via ProteomeXchange.

Title	Infection by wMel Wolbachia alters female post-mating behaviors and physiology in the dengue vector mosquito Aedes aegypti
Description	Globally invasive Aedes aegypti mosquitoes disseminate numerous arboviruses that impact human health. One promising method to control Ae. aegypti populations is transinfection with the intracellular bacterium Wolbachia pipientis, a symbiont that naturally infects ~40-52% of insects but is normally absent from Ae. aegypti. Transinfection of Ae. aegypti with the wMel Wolbachia strain induces cytoplasmic incompatibility (CI), allowing infected individuals to rapidly invade native populations. Further, wMel Wolbachia-infected females display refractoriness to medically relevant arboviruses. Thus, wMel Wolbachia-infected Ae. aegypti are being released in several areas to replace native populations, thereby suppressing disease transmission by this species. Wolbachia is reported to have minimal effects on Ae. aegypti fertility, but its influence on other reproductive processes is unknown. Female insects undergo several post-mating physiological and behavioral changes required for optimal fertility. Post-mating responses (PMRs) in female insects are typically elicited by receipt of male seminal fluid proteins (SFPs) transferred with sperm during mating, but can be modified by other factors, such as adult age, nutritional status, and microbiome composition. To assess how Wolbachia infection influences Ae. aegypti female PMRs, we collected wMel Wolbachia-infected Ae. aegypti from the field in Medellín, Colombia and introduced the bacterium into our laboratory strain. We found that Wolbachia influences female fecundity, fertility, and re-mating incidence. Further, we observed that Wolbachia significantly extends longevity of virgin females. Changes in female PMRs are not due to defects in sperm transfer by infected males, or sperm storage by infected females. Using proteomic methods to examine the seminal proteome of infected males, we found that Wolbachia infection has a moderate effect on SFP composition. However, we identified 125 Wolbachia proteins that are paternally transferred to females by infected males. Surprisingly, the CI factor proteins (Cifs), were not detected in the ejaculates of Wolbachia-infected males. Our findings indicate that Wolbachia infection of Ae. aegypti alters female post-mating responses, potentially influencing control programs that utilize Wolbachia-infected individuals.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:07:29.299.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043965
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	steve dorus
SpeciesList	scientific name: Aedes aegypti aegypti; NCBI TaxID: 1424507;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-20 12:52:31	ID requested
1	2023-10-24 07:55:17	announced
2	2023-11-14 09:06:23	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:07:30	announced	2024-10-22: Updated project metadata.

10.6019/PXD043965;

Osorio J, Villa-Arias S, Camargo C, Ram, í, rez-S, á, nchez LF, Barrientos LM, Bedoya C, R, ú, a-Uribe G, Dorus S, Alfonso-Parra C, Avila FW, wMel Wolbachia alters female post-mating behaviors and physiology in the dengue vector mosquito Aedes aegypti. Commun Biol, 6(1):865(2023) [pubmed]

10.1038/s42003-023-05180-8;

submitter keyword: female reproductive tract, seminal fluid, sperm,reproduction

Steve Dorus
contact affiliation	Center for Reproductive Evolution Syracuse University
contact email	sdorus@syr.edu
lab head
steve dorus
contact affiliation	Syracuse University
contact email	sdorus@syr.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043965

PRIDE project URI

• PRIDE
  1. PXD043965
     1. Label: PRIDE project
     2. Name: Infection by wMel Wolbachia alters female post-mating behaviors and physiology in the dengue vector mosquito Aedes aegypti