PXD043956 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Multimode Chromatography-based Techniques for High Purity Isolation of Extracellular Vesicles from Human Blood Plasma |
| Description | Extracellular vesicles (EVs) play a pivotal role in various biological pathways, such as immune responses and the progression of diseases, including cancer. However, it is challenging to isolate EVs at high purity from blood plasma and other biofluids due to their low abundance compared to more predominant biomolecular species such as lipoprotein particles and free protein complexes. Ultracentrifugation-based EV isolation, the current gold standard technique, cannot overcome this challenge due to the similar densities of such species. We developed several novel approaches to enrich EVs from plasma while depleting contaminating molecular species using multimode chromatography-based strategies. On average, we identified 716±68 proteins and 1054±35 proteins in EV isolates from 100 µL of plasma using multimode chromatography- and ultracentrifugation-based techniques, respectively. The developed methods resulted in similar EV isolates purity, providing significant advantages in simplicity, throughput, scalability, and applicability for various downstream analytical and potential clinical applications |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-08-04 |
| AnnouncementXML | Submission_2025-08-03_17:54:16.222.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Alan Zimmerman |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-07-20 08:20:47 | ID requested | |
| ⏵ 1 | 2025-08-03 17:54:16 | announced | |
Publication List
Keyword List
| submitter keyword: size exclusion chromatography, mass spectrometry, multimode chromatography, plasma,Extracellular vesicles |
Contact List
| Alexander Ivanov |
|---|
| contact affiliation | Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University |
| contact email | a.ivanov@northeastern.edu |
| lab head | |
| Alan Zimmerman |
|---|
| contact affiliation | Northeastern University |
| contact email | zimmerman.alan@northeastern.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043956
- Label: PRIDE project
- Name: Multimode Chromatography-based Techniques for High Purity Isolation of Extracellular Vesicles from Human Blood Plasma
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