PXD043941 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The interactome of the UapA transporter reveals putative new players in anterograde membrane cargo trafficking: UapA-DYDY TurboID |
Description | Neosynthesized plasma membrane (PM) proteins co-translationally translocate to the ER, concentrate at regions called ER-exit sites (ERes) and pack into COPII secretory vesicles which are sorted to the early-Golgi through membrane fusion. Following Golgi maturation, membrane cargoes reach the late-Golgi, from where they exit in clathrin-coated vesicles destined to the PM, directly or through endosomes. Post-Golgi membrane cargo trafficking also involves the cytoskeleton and the exocyst. The Golgi-dependent secretory pathway is thought to be responsible for the trafficking of all major membrane proteins. However, our recent findings in Aspergillus nidulans showed that several plasma membrane cargoes, such as transporters and receptors, follow a sorting route that seems to bypass Golgi functioning. To gain insight on membrane trafficking and specifically Golgi-bypass, here we used proximity dependent biotinylation (PDB) coupled with data-independent acquisition mass spectrometry (DIA-MS) for identifying transient interactors of the UapA transporter. Our assays, which included proteomes of wild-type and mutant strains affecting ER-exit (UapA-DYDY TurboID) or endocytosis, identified both expected and novel interactions that might be physiologically relevant to UapA trafficking. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-21 |
AnnouncementXML | Submission_2023-11-21_08:08:21.578.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Martina Samiotaki |
SpeciesList | scientific name: Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans); NCBI TaxID: 227321; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-20 01:19:30 | ID requested | |
⏵ 1 | 2023-11-21 08:08:22 | announced | |
Publication List
Georgiou X, Dimou S, Diallinas G, Samiotaki M, The interactome of the UapA transporter reveals putative new players in anterograde membrane cargo trafficking. Fungal Genet Biol, 169():103840(2023) [pubmed] |
10.1016/j.fgb.2023.103840; |
Keyword List
submitter keyword: ER-exit |
COPII |
COPI |
traffic |
fungi |
A. nidulans, Golgi-bypass |
UapA |
Proximity Dependent Biotinylation |
proteomics |
Contact List
George Diallinas |
contact affiliation | 1Department of Biology, National and Kapodistrian University of Athens, Panepistimioupolis, Athens, 15784, Greece. 2Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion, 70013, Greece. |
contact email | diallina@biol.uoa.gr |
lab head | |
Martina Samiotaki |
contact affiliation | Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece |
contact email | samiotaki@fleming.gr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043941
- Label: PRIDE project
- Name: The interactome of the UapA transporter reveals putative new players in anterograde membrane cargo trafficking: UapA-DYDY TurboID