PXD043878-1

PXD043878 is an original dataset announced via ProteomeXchange.

Title	Utilization of Stability-based Proteomics for the Investigation of Structured RNA-protein Interactions
Description	The stability of proteins from rates of oxidation (SPROX), thermal protein profiling (TPP) methods as well as triple helix pulldowns with LC-MS/MS readouts are used to identify the protein targets of three RNA ligands, the MALAT1 triple helix (TH), a viral stem loop (SL), and an unstructured RNA (PolyU) in LNCaP nuclear lysate. This work establishes a novel platform for the global discovery and interrogation of RNA-protein interactions that is generalizable to numerous biological contexts and RNA targets.
HostingRepository	PRIDE
AnnounceDate	2024-01-31
AnnouncementXML	Submission_2024-01-31_15:56:53.327.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Morgan Bailey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-18 20:05:38	ID requested
⏵ 1	2024-01-31 15:56:53	announced
2	2024-10-22 06:28:09	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Thermodynamics, Thermal Denaturation, Chemical Denaturation, RNA, RNA-Protein Interactions, Proteomics

Dr. Michael C. Fitzgerald
contact affiliation	PI of the Fitzgerald lab at Duke University Department of Chemistry
contact email	michael.c.fitzgerald@duke.edu
lab head
Morgan Bailey
contact affiliation	Duke University Department of Chemistry
contact email	morgan.a.bailey@duke.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD043878

PRIDE project URI

• PRIDE
  1. PXD043878
     1. Label: PRIDE project
     2. Name: Utilization of Stability-based Proteomics for the Investigation of Structured RNA-protein Interactions