PXD043854-1
PXD043854 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The proteins identified from Staphylococcus aureus-derived extracellular vesicles (SaEVs) |
Description | Staphylococcus aureus strain ATCC 1718 was inoculated at 0.5% in 400 mL of Brain Heart Infusion broth for 8 h under aerobic conditions. The supernatant was collected by centrifugation twice at 5,000 x g, 4°C for 20 min, filtrated through a 0.45 µm filter to remove all remaining bacterial cells. S. aureus-derived extracellular vesicles (SaEVs) in culture supernatant were precipitated by ultracentrifugation at 100,000 x g, 4°C for 90 min using a Himac CP80WX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After washing with ice-cold PBS, the pellet was suspended and applied to discontinuous iodixanol gradient ultracentrifugation. Layers of 40%, 20%, 10%, and 5% iodixanol in 0.25 M sucrose/10 mM Tris, pH 7.5 were prepared using OptiPrep™ [60% (w/v) iodixanol; Sigma Aldrich, St. Louis, MO]. After centrifugation at 100,000 x g, 4°C for 16 h, the substances in all six fractions (Fr1-Fr6) were collected, diluted in PBS, and precipitated by ultracentrifugation at 100,000 × g, 4°C for 2 h. The pellets were then washed and resuspended in an appropriate volume of ice-cold PBS. |
HostingRepository | jPOST |
AnnounceDate | 2024-01-30 |
AnnouncementXML | Submission_2024-01-29_18:00:36.179.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yota Tatara |
SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
ModificationList | L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine; phosphorylated L-histidine; O-phospho-L-serine |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-07-17 18:50:41 | ID requested | |
⏵ 1 | 2024-01-29 18:00:37 | announced |
Publication List
Subsomwong P, Teng W, Ishiai T, Narita K, Sukchawalit R, Nakane A, Asano K, Extracellular vesicles from Staphylococcus aureus promote the pathogenicity of Pseudomonas aeruginosa. Microbiol Res, 281():127612(2024) [pubmed] |
Keyword List
submitter keyword: Staphylococcus aureus, extracellular vesicles, proteome |
Contact List
Krisana Asano | |
---|---|
lab head | |
Yota Tatara | |
contact affiliation | Hirosaki University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002251/ |