PXD043836-1

PXD043836 is an original dataset announced via ProteomeXchange.

Title	Species-specific metabolic reprogramming in human and mouse microglia during inflammatory pathway induction
Description	Metabolic reprogramming is a hallmark of the immune cells in response to inflammatory stimuli. This metabolic process involves a switch from oxidative phosphorylation (OXPHOS)to glycolysis, or alterations in other metabolic pathways. However, most of the experimental findings have been acquired in murine immune cells and little is known about the metabolic reprogramming of human microglia. In this study, we investigated the transcriptomic and metabolic profiles of mouse and iPSC-derived human microglia challenged with the TLR4 agonist LPS. We found that both species displayed a metabolic shift and an overall increased glycolytic gene signature in response to LPS treatment. The metabolic reprogramming was characterized by the upregulation of hexokinases in mouse microglia and phosphofructokinases in human microglia. This study provides the first direct comparison of energy metabolism between mouse and human microglia, highlighting the species-specific pathways involved in immunometabolism and the importance of considering these differences in translational research.
HostingRepository	PRIDE
AnnounceDate	2023-09-02
AnnouncementXML	Submission_2023-09-02_00:33:50.351.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Justina ClarindaWolters
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-17 03:09:50	ID requested
⏵ 1	2023-09-02 00:33:50	announced
2	2024-10-22 06:02:26	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: human, LPS treatment, mouse,microglia

Dr. Justina ClarindaWolters
contact affiliation	Laboratory of Pediatrics, Section Systems Medicine of Metabolism and Signaling, Faculty of Medical Sciences, University of Groningen, University Medical Center Groningen, Groningen,the Netherlands
contact email	j.c.wolters@umcg.nl
lab head
Justina ClarindaWolters
contact affiliation	UMCG
contact email	j.c.wolters@umcg.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043836
     1. Label: PRIDE project
     2. Name: Species-specific metabolic reprogramming in human and mouse microglia during inflammatory pathway induction