PXD043808-1

PXD043808 is an original dataset announced via ProteomeXchange.

Title	The transcription factor AP2XI-2 is a key negative regulator of Toxoplasma gondii merogony
Description	Commitment to and completion of sexual development are essential for Toxoplasma gondii to produce oocysts in the intestine of the feline host. Understanding of the molecular mechanism responsible for sexual commitment is extremely limited due to the lack of model systems. Here, we show that the transcription factors AP2XI-2 and AP2XII-1 associated with the epigenetic repressors MORC/HDAC3 complex are constitutively expressed in both tachyzoite and bradyzoite stages but not in the merozoite stage. Depletion of AP2XI-2 or AP2XII-1 elicits the expression of genes specific to merozoites, but they play different roles in the merogony process. Depletion of AP2XI-2 in type II Pru strain induced parasites to undergo merogony and produce mature merozoites in alkaline medium but not in neutral medium, whereas the AP2XII-1 depleted Pru strain underwent several rounds of schizogony and produced merozoites in neutral medium and more markedly under alkaline conditions. Furthermore, we show that two AP2XI-2 interacting proteins are also involved in repressing merozoite programming. Overall, these findings indicate that the merozoite primed pre-sexual commitment is controlled by an intricate regulatory network and that AP2XI-2 or AP2XII-1 depleted parasites can be used as a model to study the merogony in vitro.
HostingRepository	PRIDE
AnnounceDate	2023-12-20
AnnouncementXML	Submission_2023-12-19_18:30:46.434.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JinLei Wang
SpeciesList	scientific name: Toxoplasma gondii ME49; NCBI TaxID: 508771;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-15 06:49:19	ID requested
⏵ 1	2023-12-19 18:30:46	announced

Dataset with its publication pending

submitter keyword: Toxoplasma gondii

transcription factors

AP2XI-2

merogony

pre-sexual development

Jinlei Wang
contact affiliation	College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
contact email	wangjinlei90@126.com
lab head
JinLei Wang
contact affiliation	Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
contact email	wangjinlei90@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD043808

PRIDE project URI

• PRIDE
  1. PXD043808
     1. Label: PRIDE project
     2. Name: The transcription factor AP2XI-2 is a key negative regulator of Toxoplasma gondii merogony