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PXD043799-1

PXD043799 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted mass spectrometry analyses of somatic mutations in colorectal cancer specimens using differential ion mobility
DescriptionIdentification of K-Ras and B-Raf mutations in colorectal cancer (CRC) is essential to predict patients' response to anti-EGFR therapy and formulate appropriate therapeutic strategies to improve prognosis and survival. Here, we combined parallel reaction monitoring (PRM) with high-field asymmetric waveform ion mobility (FAIMS) to enhance mass spectrometry sensitivity and improve the identification of low-abundance K-Ras and B-Raf mutations in biological samples without immunoaffinity enrichment. In targeted LC-MS/MS analyses, FAIMS reduced the occurrence of interfering ions and enhanced precursor ion purity, resulting in a three-fold improvement in the detection limit for K-Ras and B-Raf mutated peptides. In addition, ion mobility separation of isomeric peptides using FAIMS facilitated the unambiguous identification of K-Ras G12D and G13D peptides. The application of targeted LC-MS/MS analyses using FAIMS is demonstrated for the detection and quantitation of B-Raf V600E, K-Ras G12D, G13D, and G12V in CRC cell lines and primary specimens.
HostingRepositoryPRIDE
AnnounceDate2023-08-14
AnnouncementXMLSubmission_2023-08-14_04:49:52.153.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEricBonneil
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-14 13:53:28ID requested
12023-08-14 04:49:52announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mass spectrometry, parallel reaction monitoring (PRM), BRAF,High field asymmetric waveform ion mobility (FAIMS), colorectal cancer mutations, KRAS
Contact List
PierreThibault
contact affiliationIRIC-Université de Montréal
contact emailpierre.thibault@umontreal.ca
lab head
EricBonneil
contact affiliationProteomic Platform
contact emaileric.bonneil@umontreal.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
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