PXD043783-1

PXD043783 is an original dataset announced via ProteomeXchange.

Title	Proteomic and phosphoproteomic profiling of D816V- and V560G-mutant KIT cells
Description	The CD117 mast/stem cell growth factor receptor tyrosine kinase (KIT) is critical for haematopoiesis, melanogenesis, and stem cell maintenance. KIT is commonly activated by mutation in cancers including acute myeloid leukaemia, melanoma, and gastrointestinal stromal tumours (GISTs). The kinase and the juxtamembrane domains of KIT are mutation hotspots; with the kinase domain mutation D816V common in leukaemia, and the juxtamembrane domain mutation V560G common in GISTs. Given the importance of mutant KIT signalling in cancer, we have conducted a proteomic and phosphoproteomic analysis of D816V- and V560G-KIT mutations, using an FDCP1 isogenic cell line model.
HostingRepository	PRIDE
AnnounceDate	2025-06-19
AnnouncementXML	Submission_2025-06-18_21:27:10.970.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043783
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nicole Verrills
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-14 02:25:07	ID requested
⏵ 1	2025-06-18 21:27:11	announced

10.1002/pmic.202300309;

Murray HC, Miller K, Dun MD, Verrills NM, Pharmaco-phosphoproteomic analysis of cancer-associated KIT mutations D816V and V560G. Proteomics, 24(9):e2300309(2024) [pubmed]

10.6019/PXD043783;

submitter keyword: proteomics,Phosphoproteomics, KIT, AML

Nikki Verrills
contact affiliation	School of Biomedical Sciences and Pharmacy, College of Health, Medicine and Wellbeing, University of Newcastle, Callaghan, and Precision Medicine Research Program, Hunter Medical Research Institute, NSW, 2308, Australia
contact email	Nikki.Verrills@newcastle.edu.au
lab head
Nicole Verrills
contact affiliation	School of Biomedical Sciences and Pharmacy Faculty of Health and Medicine, University of Newcastle, and Cancer Research Program, Hunter Medical Research Institute, Callaghan, NSW, 2308, Australia
contact email	Nikki.Verrills@newcastle.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD043783

PRIDE project URI

• PRIDE
  1. PXD043783
     1. Label: PRIDE project
     2. Name: Proteomic and phosphoproteomic profiling of D816V- and V560G-mutant KIT cells