PXD043770-1

PXD043770 is an original dataset announced via ProteomeXchange.

Title	Mapping naturally presented T-cell antigens in medulloblastoma based on integrative multi-omics
Description	Medulloblastoma is the most frequent malignant primary brain tumor in children. Despite recent advances in integrated genomics, the prognosis in children with high-risk medulloblastoma remains devastating, and new tumor-specific therapeutic approaches are needed. Here, we present an atlas of naturally presented T-cell antigens in medulloblastoma. We mapped the human leukocyte antigen (HLA)-presented peptidomes of 28 tumors and performed comparative immunopeptidome profiling against an in-house benign database. Medulloblastoma proved to be a rich source of novel tumor-associated antigens, naturally presented on HLA class I and II molecules. Remarkably, most tumor-associated peptides and proteins were subgroup-specific, whereas shared presentation among all subgroups of medulloblastoma (WNT, SHH, Group 3 and Group 4) was rare. Functional testing of top-ranking novel candidate antigens demonstrated the induction of peptide-specific T-cell responses, supporting their potential for T-cell immunotherapy. This study is an in-depth mapping of naturally presented T-cell antigens in medulloblastoma. Integration of immunopeptidomics, transcriptomics, and epigenetic data led to the identification of a large set of actionable targets that can be further used for the translation into the clinical setting by facilitating the informed design of immunotherapeutic approaches to children with medulloblastoma.
HostingRepository	PRIDE
AnnounceDate	2024-08-24
AnnouncementXML	Submission_2024-08-24_04:24:16.397.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonas Scheid
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-13 11:11:22	ID requested
⏵ 1	2024-08-24 04:24:16	announced
2	2024-10-22 06:56:23	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Medulloblastoma, Immunopeptidomics, RNA-seq

Marian Christoph
contact affiliation	Laboratory of Molecular Neuro-Oncology, Department of Neurology, Clinical Neuroscience Center, University Hospital and University of Zurich, Frauenklinikstrasse 26, 8091 Zurich, Switzerland Department of Neurosurgery, Cantonal Hospital St.Gallen, Rorschacher Strasse 95, 9007 St.Gallen, Switzerland
contact email	marian.neidert@kssg.ch
lab head
Jonas Scheid
contact affiliation	Department of Peptide-based Immunotherapy, University and Universityhospital of Tübingen Quantitative Biology Center, University of Tübingen
contact email	jonas.scheid@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043770
     1. Label: PRIDE project
     2. Name: Mapping naturally presented T-cell antigens in medulloblastoma based on integrative multi-omics