PXD043762-1
PXD043762 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A thylakoid biogenesis BtpA protein is required for the initial step of tetrapyrrole biosynthesis in cyanobacteria |
| Description | Biogenesis of the photosynthetic apparatus requires complicated molecular machinery, individual components of which are either poorly characterized or unknown. The BtpA protein has been described as a factor required for the stability of Photosystem I (PSI) in cyanobacteria; however, how the BtpA stabilized PSI remains unexplained. To clarify the role of BtpA, we constructed and characterized the btpA-null mutant (btpA) in the cyanobacterium Synechocystis sp. PCC 6803. The mutant contained only ~ 1% of chlorophyll and nearly no thylakoid membranes. However, this strain, growing only in the presence of glucose, was genetically unstable and readily generate suppressor mutations that restore the photoautotrophy. Two suppressor mutations were mapped into the hemA gene encoding glutamyl-tRNA reductase (GluTR) - the first enzyme of tetrapyrrole biosynthesis. Indeed, the GluTR was not detectable in the btpA mutant and the suppressor mutations restored biosynthesis of tetrapyrroles and photoautotrophy by increased GluTR expression or by improved GluTR stability/processivity. We further demonstrated that GluTR associates with a large BtpA oligomer and that BtpA is required for the stability of GluTR. Our results show that the BtpA protein is involved in the biogenesis of photosystems on the level of regulation of tetrapyrrole biosynthesis. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-10-27 |
| AnnouncementXML | Submission_2023-10-27_06:01:01.992.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Peter Konik |
| SpeciesList | scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-07-13 07:24:32 | ID requested | |
| ⏵ 1 | 2023-10-27 06:01:02 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: BtpA, Glutamyl-tRNA reductase, Cyanobacteria, Synechocystis sp. PCC 6803, Tetrapyrrole biosynthesis |
Contact List
| Roman Sobotka | |
|---|---|
| contact affiliation | 1Laboratory of Photosynthesis, Centre Algatech, Institute of Microbiology, The Czech Academy of Sciences, 37901 Trebon; Faculty of Science, University of South Bohemia, 37005, Ceske Budejovice |
| contact email | sobotka@alga.cz |
| lab head | |
| Peter Konik | |
| contact affiliation | University of South Bohemia, Ceske Budejovice |
| contact email | konik@prf.jcu.cz |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092431/ |
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