PXD043746-1

PXD043746 is an original dataset announced via ProteomeXchange.

Title	Dynorphin-sensing two-component systems ParRS and CprRS in Pseudomonas aeruginosa pathogenesis
Description	The widespread presence of antibiotic-resistant bacteria in the environment has been recognized as an important emerging environmental contaminant. Hospital wards, as a special public indoor environment, are of great concern for the risks associated with this emerging environmental contaminant. Pseudomonas aeruginosa, a common nosocomial bacterium, is a contamination risk in the hospital environment due to its drug resistance and transmission of virulence factors. Notably, the antimicrobial peptide-sensing two-component system (TCS) ParRS and CprRS have been implicated in dynorphin-induced signaling, but the underlying Manuscript2 mechanism has remained elusive. In this study, we performed proteomic analysis to systematically investigate the contributions of ParRS and CprRS to P. aeruginosa pathogenesis and dynorphin-induced resistance to polymyxins. Additionally, we characterized the significance of the extracellular sensor domains of ParS and CprS in dynorphin perception. Furthermore, through structural biology, we identified additional TCS sensors with similar extracellular domain conformations, which also directly interacted with dynorphin in vitro. This suggests convergent evolution in different bacterial TCSs for host-derived synthetic peptide signal transmitting. Our findings establish a link between CAMPs resistance associated TCSs and virulence regulation of common nosocomial bacteria. This further illustrates the danger of this emerging contaminant for the environment and humans.
HostingRepository	PRIDE
AnnounceDate	2024-07-04
AnnouncementXML	Submission_2024-07-03_19:19:05.531.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	hanzhong feng
SpeciesList	scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-13 01:42:33	ID requested
⏵ 1	2024-07-03 19:19:05	announced

10.1016/J.JHAZMAT.2023.132512;

submitter keyword: Pseudomonas aeruginosa

ParRS

CprRS

resistance

dynorphin

Yomngxing He
contact affiliation	Lanzhou University, Lanzhou, Gansu, China
contact email	heyx@lzu.edu.cn
lab head
hanzhong feng
contact affiliation	Lanzhou University
contact email	fenghzh19@lzu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD043746

PRIDE project URI

• PRIDE
  1. PXD043746
     1. Label: PRIDE project
     2. Name: Dynorphin-sensing two-component systems ParRS and CprRS in Pseudomonas aeruginosa pathogenesis