PXD043738-3

PXD043738 is an original dataset announced via ProteomeXchange.

Title	Time-Dependent Effects of Anesthetic Administration on the Rat Hypothalamus and Cerebral Cortex Peptidome
Description	Prohormone-derived neuropeptides act as cell-cell signaling molecules to mediate a wide variety of biological processes in the animal brain. Mass spectrometry-based peptidomic experiments are valuable approaches to gain insight into the dynamics of individual peptides under different physiological conditions or experimental treatments. However, the use of anesthetics during animal procedures may confound experimental peptide measurements, especially in the brain where anesthetics act. Here, we investigate the effect of the commonly used anesthetics isoflurane and sodium pentobarbital on the peptide profile in the rodent hypothalamus and cerebral cortex, as assessed by label-free quantitative peptidomics. Our results showed that neither anesthetic dramatically alters peptide levels, although extended isoflurane exposure did cause changes in a small number prohormone-derived peptides in the cerebral cortex. Overall, our results demonstrate that acute use of anesthetics can be used in peptidomic experiments of the hypothalamus and cerebral cortex without dramatic confounding of experimental results.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:25.722.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043738
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	James Checco
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	2-pyrrolidone-5-carboxylic acid; amidated residue; phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Xevo G2-XS QTof

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-12 20:04:51	ID requested
1	2023-10-25 14:50:50	announced
2	2023-11-14 07:52:30	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:13:26	announced	2024-10-22: Updated project metadata.

10.6019/PXD043738;

10.1021/ACSCHEMNEURO.3C00499;

submitter keyword: Keywords Peptidomics, hypothalamus, neuropeptides, anesthetics, cerebral cortex

James Checco
contact affiliation	University of Nebraska-Lincoln
contact email	checco@unl.edu
lab head
James Checco
contact affiliation	University of Nebraska-Lincoln
contact email	checco@unl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043738
     1. Label: PRIDE project
     2. Name: Time-Dependent Effects of Anesthetic Administration on the Rat Hypothalamus and Cerebral Cortex Peptidome