PXD043730-1

PXD043730 is an original dataset announced via ProteomeXchange.

Title	Poglut2/3 double knockout in mice results in neonatal lethality with reduced levels of fibrillin in lung tissues
Description	Fibrillins, which are extensively O-glucosylated by POGLUT2 and 3, are crucial for development and cardiopulmonary function. Using primary dermal fibroblasts derived from our POGLUT 2 and 3 double knockout mouse model combined with proteomics analyses, we show that loss of O-glucose modifications on fibrillin causes compositional changes in the extracellular environment compared to controls. Notably, a 30-50% reduction in the abundance of FBN1 and 2 was found in media samples whereas an 85% reduction was found in the extracellular matrix. Other proteins in the extracellular matrix also exhibited changes in abundance in the double knockout samples compared to wild type, but it is still unclear if these changes are simply secondary to the significant reduction in fibrillins.
HostingRepository	PRIDE
AnnounceDate	2024-06-10
AnnouncementXML	Submission_2024-06-10_13:52:58.523.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Williamson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-12 13:54:43	ID requested
⏵ 1	2024-06-10 13:52:59	announced

Neupane S, Williamson DB, Roth RA, Halabi CM, Haltiwanger RS, Holdener BC, Poglut2/3 double knockout in mice results in neonatal lethality with reduced levels of fibrillin in lung tissues. J Biol Chem, 300(7):107445(2024) [pubmed]

10.1016/j.jbc.2024.107445;

submitter keyword: Mouse, Q Exactive, dermal fibroblasts, secretome, extracellular matrix

Robert S.
contact affiliation	University of Georgia Complex Carbohydrate Research Center Department of Biochemistry and Molecular Biology
contact email	rhalti@uga.edu
lab head
Daniel Williamson
contact affiliation	University of Georgia
contact email	dw49986@uga.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043730
     1. Label: PRIDE project
     2. Name: Poglut2/3 double knockout in mice results in neonatal lethality with reduced levels of fibrillin in lung tissues