PXD043727 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of proteome of THP-1 macrophages incubated with Paracoccidioides brasiliensis yeast cells alive or heat-killed |
Description | Paracoccidioides spp. is the etiological agent of Paracoccidioidomycosis (PCM), a systemicinfection with wide distribution in Latin America. Macrophages are very important cells during the response to infection by Paracoccidoides brasiliensis and understanding the interaction between the fungus and immune cells is very relevant for understanding the disease. In this study, we performed a proteomic analysis to assess the consequences of P. brasiliensis yeast infection on the THP-1 macrophage proteome and to verify whether there are differences between the proteome of cells infected with the live fungus. We identified 443 upregulated and 2247 downregulated proteins in macrophages infected with live P. brasiliensis yeasts, compared to uninfected macrophages. Proteins differentially expressed in cells infected are related to metabolism and energy production, protein synthesis and processing, transcription, cell cycle, DNA processing, cell signaling, oxidative stress, immune response, among other processes . Proteomic analysis revealed that P. brasiliensis, causes metabolic alterations in infected cells, drastically affecting energy production pathways. In addition, macrophages showed many upregulated, mostly downregulated, immune system proteins. Thus, the present work contributes to elucidate the changes that occur in immune cells in response to infection by P. brasiliensis and may help to better understand PCM. |
HostingRepository | PRIDE |
AnnounceDate | 2023-10-23 |
AnnouncementXML | Submission_2023-10-23_11:15:39.216.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | CeliaSoares |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monomethylated residue; carbamoylated residue; monohydroxylated residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-12 12:20:46 | ID requested | |
⏵ 1 | 2023-10-23 11:15:39 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: P. brasiliensis |
Proteomic analysis |
Metabolic reprogramming |
Immunometabolism |
Contact List
Celia Maria AlmeidaSoares |
contact affiliation | Universidade Federal de Goias |
contact email | cmasoares@gmail.com |
lab head | |
CeliaSoares |
contact affiliation | UFG |
contact email | hplcmslbm@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043727
- Label: PRIDE project
- Name: Proteomic analysis of proteome of THP-1 macrophages incubated with Paracoccidioides brasiliensis yeast cells alive or heat-killed