PXD043721-1

PXD043721 is an original dataset announced via ProteomeXchange.

Title	A Macrophage-Collagen Fragment Axis Mediates Subcutaneous Adipose Tissue Remodeling
Description	Efficient collagen degradation is essential for adaptive subcutaneous adipose tissue (SAT) expansion that protects against ectopic lipid deposition during weight gain. Here, we aimed to further define the mechanism for this collagenolytic process. We show that loss of collagen type-1 (CT1) and increased CT1-fragment levels in expanding SAT are associated with proliferation of resident M2-like macrophages that display increased CD206-mediated engagement in collagen endocytosis compared to chow-fed controls. Blockage of CD206 during acute diet-induced weight gain leads to SAT CT1-fragment accumulation associated with elevated inflammation and fibrosis markers. Moreover, the collagen endocytosis engagement of SAT macrophages is dramatically reduced in obesity along with elevated levels of short collagen fragments. Finally, we show that such fragments provoke M2-macrophage proliferation and fibroinflammatory changes in vitro. Thus, our data delineate the importance of a macrophage-collagen axis in physiological SAT expansion. Further understanding of this process can define novel targets in obesity-related disorders.
HostingRepository	PRIDE
AnnounceDate	2024-06-16
AnnouncementXML	Submission_2024-06-16_01:23:28.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Fuchs
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-12 07:58:20	ID requested
⏵ 1	2024-06-16 01:23:29	announced

Vuji, č, i, ć M, Broderick I, Salmantabar P, Perian C, Nilsson J, Sihlbom Wallem C, Wernstedt Asterholm I, A macrophage-collagen fragment axis mediates subcutaneous adipose tissue remodeling in mice. Proc Natl Acad Sci U S A, 121(6):e2313185121(2024) [pubmed]

10.1073/pnas.2313185121;

submitter keyword: collagen fragments, macrophages, fibrosis, ECM-remodeling, collagen endocytosis,Subcutaneous adipose tissue, CD206

Carina Sihlbom
contact affiliation	Proteomics Core Facility, University of Gothenburg
contact email	carina.sihlbom@gu.se
lab head
Johannes Fuchs
contact affiliation	Gothenburg University
contact email	johannes.fuchs@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD043721

PRIDE project URI

• PRIDE
  1. PXD043721
     1. Label: PRIDE project
     2. Name: A Macrophage-Collagen Fragment Axis Mediates Subcutaneous Adipose Tissue Remodeling