PXD043700-2

PXD043700 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry analysis of synaptic proteome of adult Fmr1 KO and wild-type mice.
Description	Fragile X syndrome (FXS) is the most common monogenetic cause of inherited intellectual disability and autism in humans. One of the well-characterized molecular phenotypes of Fmr1 KO mice, a model of FXS, is increased translation of synaptic proteins. Although this upregulation stabilizes in the adulthood, abnormalities during the critical period of plasticity have long-term effects on circuit formation and synaptic properties. Using high-resolution quantitative proteomics of synaptoneurosomes isolated from the adult, developed brains of Fmr1 KO mice, we show differential abundance of proteins regulating postsynaptic receptor activity of glutamatergic synapse. This work includes proteomic dataset that was acquired and analyzed to quantify changes in the abundance of proteins in synaptoneurosomes (basal state, unstimulated and in vitro NMDA-R stimulated) isolated from Fmr1 KO mice and their wild-type littermates.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:27:17.587.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-12 01:10:42	ID requested
1	2024-01-26 10:22:08	announced
⏵ 2	2024-10-22 06:27:19	announced	2024-10-22: Updated project metadata.

Chojnacka M, Beroun A, Magnowska M, Stawikowska A, Cysewski D, Milek J, Dziembowska M, Kuzniewska B, Impaired synaptic incorporation of AMPA receptors in a mouse model of fragile X syndrome. Front Mol Neurosci, 16():1258615(2023) [pubmed]

10.3389/fnmol.2023.1258615;

submitter keyword: brain,synaptoneurosomes, Fmr1 KO mice

Magdalena Dziembowska
contact affiliation	University of Warsaw, Warsaw, Poland
contact email	m.dziembowska@cent.uw.edu.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043700
     1. Label: PRIDE project
     2. Name: Mass spectrometry analysis of synaptic proteome of adult Fmr1 KO and wild-type mice.