PXD043650-1

PXD043650 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics identifies targets of Mos-MAPK regulating translation and spindle organization in oocyte meiosis
Description	The Mos kinase is a constitutive activator of the ERK/MAPK pathway exclusively expressed during oocyte meiosis, mediating key meiotic functions across animal species. While a few of its downstream effectors have been studied in some detail, molecular targets under the control of Mos-MAPK have not yet been identified systematically. Here, we combined live-cell microscopy of starfish oocytes to characterize the cellular phenotypes caused by Mos-MAPK inhibition with phosphoproteomic analysis of synchronous oocyte populations at critical transitions. This revealed a large set of proteins involved in regulation of translation through the CPE-element binding protein CPEB. Our data indicate cyclin B to be a main target of this regulation driving the second meiotic division. A second large group of phospho-proteins identified are regulators of the actin and microtubule cytoskeleton, with a prominent subset of regulators of centrosomal microtubule nucleation. Indeed, we show that Mos-MAPK inhibition increases the size of microtubule asters and promotes separation of spindle poles in anaphase, turning meiotic spindles mitotic-like. We thus identified core molecular modules downstream of Mos-MAPK controlling meiotic functions essential for haploidization and for the highly asymmetric polar body divisions. These modules are widely conserved, thus our findings will likely have general relevance for reproductive processes across species, including humans, and for understanding disease mechanisms when Mos is expressed erroneously, acting as an oncogene.
HostingRepository	PRIDE
AnnounceDate	2025-09-03
AnnouncementXML	Submission_2025-09-03_05:17:30.970.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olexandr Dybkov
SpeciesList	scientific name: Echinoderma sp.; NCBI TaxID: 2040765;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-10 09:54:07	ID requested
⏵ 1	2025-09-03 05:17:31	announced

10.1083/JCB.202312140;

submitter keyword: Phosphoproteomics, oocyte meiosis

Henning Urlaub
contact affiliation	Research Group Bioanalytical Mass Spectrometry, Max Planck Institute for Multidisciplinary Sciences, Germany; Bioanalytics, Department of Clinical Chemistry, University Medical Center Goettingen, Germany
contact email	henning.urlaub@mpinat.mpg.de
lab head
Olexandr Dybkov
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	odybkov@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043650
     1. Label: PRIDE project
     2. Name: Phosphoproteomics identifies targets of Mos-MAPK regulating translation and spindle organization in oocyte meiosis