PXD043581-1

PXD043581 is an original dataset announced via ProteomeXchange.

Title	A proteomic-based approach identifies the NEDD4-adaptor NDFIP2 as an important regulator of IFITM3
Description	IFITMs are a family of highly related interferon-induced transmembrane proteins that interfere with the processes of fusion between viral and cellular membranes and are thus endowed with broad antiviral properties. A number of studies have shown how the antiviral potency of IFITMs is highly dependent on their steady-state levels, their intracellular distribution as well as on a complex pattern of post-translational modifications, parameters that are overall tributary of a number of cellular partners. In an effort to identify additional protein partners involved in the biology of IFITMs, we have devised a proteomic-based approach based on the piggyback incorporation of IFITM3 partners into extracellular vesicles. MS analysis of the proteome of vesicles bearing or not IFITM3 identified the NDFIP2 protein adaptor protein as an important regulator of IFITM3 levels. NDFIP2 is a membrane-anchored adaptor protein of E3 ubiquitin ligases of the NEDD4 family that have been already involved in IFITM3 regulation.
HostingRepository	PRIDE
AnnounceDate	2023-10-26
AnnouncementXML	Submission_2023-10-25_23:24:24.790.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YohannCouté
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-06 05:22:15	ID requested
⏵ 1	2023-10-25 23:24:25	announced
2	2023-11-14 07:59:32	announced	2023-11-14: Updated project metadata.

submitter keyword: IFITM3, degradation, virus infection, vesicles, nanoLC-MS/MS

YohannCouté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
YohannCouté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043581
     1. Label: PRIDE project
     2. Name: A proteomic-based approach identifies the NEDD4-adaptor NDFIP2 as an important regulator of IFITM3