PXD043547-1

PXD043547 is an original dataset announced via ProteomeXchange.

Title	Dux promotes OGS-Epigenetic-MET network during early iPSC reprogramming via H3K18la recruiting Brg1
Description	The process of iPSC reprogramming involves various critical events such as somatic gene shutdown, mesenchymal-epithelial transition (MET), metabolism reprogramming, and epigenetic rewiring. These events interweave and influence each other leading to the formation of the iPSC reprogramming network, thus there appears to be a key element regulating this network. Dux has essential research value by promoting totipotency in the process of transition from ESC to 2C-like ESC and has not been discussed in depth in iPSC reprogramming process. This paper focused on the H3K18la modification linking the OGS-Epigenetic-MET network, which improved the efficiency for iPSC reprogramming via Dux overexpression. Dux promoted H3K18la via metabolism switch, and recruiting P300 through its C-terminal domain, resulting in increasing reprogramming. By using H3K18la regulators, we found the role H3K18la played in promoting MET. We performed proteomic detection of H3K18la by IP combined with MS and found that H3K18la specifically recruited Brg1 in iPSCs. During reprogramming, H3K18la and Brg1 enriched on promoters of epithelial-related genes and pluripotency-related genes. Overall, our work highlights H3K18la as a powerful trigger in the early reprogramming and reveals Dux as a regulator for increasing H3K18la. Brg1 was first demonstrated binding to H3K18la and identified as a reader of lactylation.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_16:23:55.501.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinglin Hu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-05 02:05:17	ID requested
⏵ 1	2026-02-22 16:23:56	announced

10.1093/nar/gkae183;

Hu X, Huang X, Yang Y, Sun Y, Zhao Y, Zhang Z, Qiu D, Wu Y, Wu G, Lei L, Dux activates metabolism-lactylation-MET network during early iPSC reprogramming with Brg1 as the histone lactylation reader. Nucleic Acids Res, 52(10):5529-5548(2024) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Stems Cells (B/D-HPP)

submitter keyword: H3K18la，MET,iPSC

Xinglin Hu
contact affiliation	Harbin Medical University, Histology and embryology
contact email	hxlhmu@hotmail.com
lab head
Xinglin Hu
contact affiliation	Harbin Medical University
contact email	hxlhmu@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043547
     1. Label: PRIDE project
     2. Name: Dux promotes OGS-Epigenetic-MET network during early iPSC reprogramming via H3K18la recruiting Brg1