PXD043519-2

PXD043519 is an original dataset announced via ProteomeXchange.

Title	The Amniotic Fluid Proteome Changes Across Gestation in Humans and Rhesus Macaques
Description	Amniotic fluid is a complex biological medium that offers mechanical protection and nutrition to the fetus, and also plays a key role in normal fetal growth, organogenesis, and potentially fetal programming. Amniotic fluid is also critically involved in longitudinally shaping the in utero milieu during pregnancy. Yet, the molecular mechanism of action by which amniotic fluid regulates fetal development is ill-defined partly due to an incomplete understanding of the evolving composition of the amniotic fluid proteome. Prior research consisting of cross-sectional studies suggests that the amniotic fluid proteome changes as pregnancy advances, yet longitudinal alterations have not been confirmed because repeated sampling is prohibitive in humans. We therefore performed serial amniocenteses at early, mid, and late gestational time-points within the same pregnancies in a rhesus macaque model. Longitudinally-collected rhesus amniotic fluid samples were paired with gestational-age matched cross-sectional human samples. Utilizing LC-MS/MS isobaric labeling quantitative proteomics, we demonstrate considerable cross-species similarity between the amniotic fluid proteomes and large scale gestational-age associated changes in protein content throughout pregnancy. This is the first study to establish a reference proteomic profile across gestation. This non-human primate model holds promise as a translational platform for amniotic fluid studies and to identify adversely affected pregnancies.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:12:44.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phillip Wilmarth
SpeciesList	scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-03 22:27:56	ID requested
1	2023-10-24 14:11:55	announced
⏵ 2	2023-11-14 09:12:46	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:13:12	announced	2024-10-22: Updated project metadata.

Shorey-Kendrick LE, Crosland BA, Spindel ER, McEvoy CT, Wilmarth PA, Reddy AP, Zientek KD, Roberts VHJ, D'Mello RJ, Ryan KS, Olyaei AF, Hagen OL, Drake MG, McCarty OJT, Scottoline BP, Lo JO, The amniotic fluid proteome changes across gestation in humans and rhesus macaques. Sci Rep, 13(1):17039(2023) [pubmed]

submitter keyword: rhesus macaque, isobaric labeling, pregnancy,Amniotic fluid, quantitative proteomics

Jamie Lo, MD MCR
contact affiliation	Division of Maternal Fetal Medicine Department of Obstetrics and Gynecology Oregon Health & Science University 3181 SW Sam Jackson Park Road, Mail Code L-458, Portland, OR, 97239, USA
contact email	loj@ohsu.edu
lab head
Phillip Wilmarth
contact affiliation	OHSU
contact email	wilmarth@ohsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043519
     1. Label: PRIDE project
     2. Name: The Amniotic Fluid Proteome Changes Across Gestation in Humans and Rhesus Macaques