PXD043492-3
PXD043492 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Deubiquitinating enzymes in human macrophages after Francisella tularensis infection |
| Description | Ubiquitination/deubiquitination belong to the most important regulatory mechanisms carried out through the attachment/removal of the ubiquitin molecule, respectively. The process is necessary not only to mark molecules for degradation, but also for example to the activation of signaling pathways leading to pro-inflammatory host response. Many intracellular pathogens, such as Francisella tularensis, have evolved mechanisms of blocking such host immune responses to escape degradation. Here, we describe that F. tularensis interferes with the host's ubiquitination system. We showed increased total activity of deubiquitinating enzymes (DUBs) in human macrophages after infection and confirmed the reduced enzymatic activities of two specific DUBs: USP10 and UCH-L5, and the increased activity of USP25. We further revealed the enrichment of these three enzymes in exosomes derived from F. tularensis-infected cells. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_06:13:16.129.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD043492 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Pavel Rehulka |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-07-03 06:36:14 | ID requested | |
| 1 | 2023-10-24 16:18:37 | announced | |
| 2 | 2023-11-14 09:12:34 | announced | 2023-11-14: Updated project metadata. |
| ⏵ 3 | 2024-10-22 06:13:17 | announced | 2024-10-22: Updated project metadata. |
Publication List
| 10.6019/PXD043492; |
| Vozandychova V, Rehulka P, Hercik K, Spidlova P, Pavlik P, Hanus J, Hadravova R, Stulik J, infection. Front Immunol, 14():1252827(2023) [pubmed] |
| 10.3389/fimmu.2023.1252827; |
Keyword List
| submitter keyword: Human, macrophage, Francisella tularensis, LC-MS, host-pathogen |
Contact List
| Jiri Stulik | |
|---|---|
| contact affiliation | Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic |
| contact email | jiri.stulik@unob.cz |
| lab head | |
| Pavel Rehulka | |
| contact affiliation | Department of Molecular Pathology and Biology FMHS UD, Trebesska 1575, CZ-50001 Hradec Kralove, Czech Republic |
| contact email | pavel.rehulka@unob.cz |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043492 |
| PRIDE project URI |
Repository Record List
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